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Updated: Jul 18, 2026
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Pan-myeloid Differentiation of Human Cord Blood Derived CD34+ Hematopoietic Stem and Progenitor Cells
Published on: August 9, 2019
Cord blood stem and progenitor cells.
Hal E Broxmeyer1, Edward Srour, Christie Orschell
1Department of Microbiology and Immunology, Medicine, and Pediatrics, Indiana University School of Medicine, Indianapolis, USA.
Methods in Enzymology
|December 5, 2006
Summary
Cord blood stem cells are crucial for treating blood disorders. Further research into their properties and manipulation will expand their therapeutic applications in medicine.
Area of Science:
- Hematology
- Stem Cell Biology
- Cellular Biology
Background:
- Cord blood is a vital source of hematopoietic stem and progenitor cells.
- These cells have been successfully used for transplantation in various malignant and nonmalignant disorders.
- Understanding these cells is key to expanding their clinical use.
Purpose of the Study:
- To review the clinical and biological properties of hematopoietic stem and progenitor cells (HSPCs) from cord blood.
- To discuss the biology of endothelial progenitor cells (EPCs).
- To present methods for detecting and quantifying these functional cells.
Main Methods:
- Enumeration of human cord blood myeloid progenitor cells (CFU-GM, BFU-E, CFU-GEMM) and their replating potential.
- Assessment of hematopoietic stem cells in vitro (LTC-ICs, CAFCs, ML-ICs) and in vivo (NOD/SCID mouse repopulating cells - SRCs).
- Quantification of high and low proliferative potential endothelial progenitor cells (EPCs).
Main Results:
- The abstract details methods for enumerating various progenitor and stem cell populations in cord blood.
- It covers both in vitro and in vivo assays for assessing stem cell function.
- Methods for quantifying endothelial progenitor cells are also presented.
Conclusions:
- Further information on cord blood stem and progenitor cells will enhance their therapeutic utility.
- Understanding and manipulating these cells can broaden their applicability in treating human diseases.
- This review provides a resource for methods to detect and quantify key functional cells in cord blood.
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