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Suitable restriction enzymes for pulsed-field gel electrophoresis analysis of Candida tropicalis
Hsiao-Hui Chou1, Chien-Shun Chiou, Kuo-Wei Chen
1Mycotic Diseases Laboratory, Center of Research and Diagnostics, Centers for Disease Control, Taipei 11561, Taiwan.
Diagnostic Microbiology and Infectious Disease
|December 5, 2006
Abstract:
This study was designed to pursue the identification of optimal restriction enzyme for pulsed-field gel electrophoresis (PFGE) analysis of Candida tropicalis. Twelve restriction enzymes were tested on C. tropicalis strains. Our results indicate that BssHII, NaeI, and RsrII were useful enzymes for PFGE analysis, and NaeI was the best for PFGE analysis of C. tropicalis isolates.