Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Antihypertensive Drugs: Angiotensin-Converting Enzyme Inhibitors01:30

Antihypertensive Drugs: Angiotensin-Converting Enzyme Inhibitors

Angiotensin-converting enzyme (ACE), a vital component of the renin-angiotensin-aldosterone system, is abundant in lung endothelial cells. ACE converts the inactive decapeptide, angiotensin I, into the active octapeptide, angiotensin II. This potent vasoconstrictor narrows blood vessels, increasing resistance to blood flow and elevating blood pressure. Angiotensin II also stimulates aldosterone production, encouraging kidney cells to reabsorb more sodium and water from urine, thereby increasing...
Regulation of the Unfolded Protein Response01:31

Regulation of the Unfolded Protein Response

Inositol-requiring kinase one or IRE1 is the most conserved eukaryotic unfolded protein response (UPR) receptor. It is a type I transmembrane protein kinase receptor with a distinctive site-specific RNase activity. As the binding mechanics of the misfolded proteins with the N-terminal domain of IRE-1 are unclear, three binding models — direct, indirect, and allosteric -- are proposed for receptor activation. Nevertheless, it is known that once a misfolded protein associates with IRE1, it...
Heart Failure Drugs: Inhibitors of Renin-Angiotensin System01:26

Heart Failure Drugs: Inhibitors of Renin-Angiotensin System

The activation of the sympathetic nervous system and the renin-angiotensin-aldosterone system (RAAS) contributes to cardiac remodeling, and inhibiting the RAAS is a pharmacological target in heart failure management. As a result, neurohumoral modulation is a crucial treatment principle for managing heart failure. This approach involves using medications like ACE inhibitors (ACEIs), angiotensin receptor blockers (ARBs), β-blockers, mineralocorticoid receptor antagonists (MRAs), and neutral...
Nitric Oxide Signaling Pathway01:28

Nitric Oxide Signaling Pathway

Nitric oxide (NO), an inorganic gas, acts as a potent second messenger in most animal and plant tissues. NO diffuses out of the cells that produce it and enters the neighboring cells to generate a downstream response. NO synthase (NOS) catalyzes NO production by the deamination of the amino acid arginine. There are three isoforms of NOS. Endothelial cells have endothelial NOS (eNOS), nerve and muscle cells have neuronal NOS (nNOS), and macrophages produce inducible NOS (iNOS) upon exposure to...
Regulated Protein Degradation02:58

Regulated Protein Degradation

It is vital to regulate the activity of enzymatic as well as non-enzymatic proteins inside the cell. This can be achieved either through creating a balance between their rate of synthesis and degradation or regulating the intrinsic activity of the protein. Both these regulation mechanisms play an essential role in the normal functioning of cells.
Protein degradation plays two important roles in the cells. It helps to protect cells from misfolded or damaged proteins before they lead to a...
Regulated Protein Degradation02:58

Regulated Protein Degradation

It is vital to regulate the activity of enzymatic as well as non-enzymatic proteins inside the cell. This can be achieved either through creating a balance between their rate of synthesis and degradation or regulating the intrinsic activity of the protein. Both these regulation mechanisms play an essential role in the normal functioning of cells.
Protein degradation plays two important roles in the cells. It helps to protect cells from misfolded or damaged proteins before they lead to a...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The association of frailty with age and lifespan in mice differs by strain and sex.

The journals of gerontology. Series A, Biological sciences and medical sciences·2026
Same author

Turning back time: a comprehensive list of interventions that decrease next-generation epigenetic aging clocks in humans.

Frontiers in genetics·2026
Same author

Corrigendum to "Oculomics and AI: The eye as a biomarker for health span" [Asia-Pac J Ophthalmol 15 (1) (2026) 100282].

Asia-Pacific journal of ophthalmology (Philadelphia, Pa.)·2026
Same author

Metabolic stress conditions dictate MAPKAPK2-dependent efficiency of MEK1/2 inhibition in colorectal carcinoma.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Epigenetic reprogramming for ocular aging and disease: Mechanisms, biomarkers, and the road to the clinic.

Progress in retinal and eye research·2026
Same author

Oculomics and AI: The eye as a biomarker for health span.

Asia-Pacific journal of ophthalmology (Philadelphia, Pa.)·2026

Related Experiment Video

Updated: Jul 18, 2026

Deacetylation Assays to Unravel the Interplay between Sirtuins (SIRT2) and Specific Protein-substrates
14:32

Deacetylation Assays to Unravel the Interplay between Sirtuins (SIRT2) and Specific Protein-substrates

Published on: February 27, 2016

Sirtuins: a conserved key unlocking AceCS activity.

Brian J North1, David A Sinclair

  • 1Department of Pathology, Paul F. Glenn Laboratories for the Biological Mechanisms of Aging, Harvard Medical School, 77 Avenue Louis Pasteur, Boston, MA 02115, USA. brian_north@hms.harvard.edu

Trends in Biochemical Sciences
|December 5, 2006
PubMed
Summary

Sirtuin enzymes control metabolism by activating acetyl-coenzyme A (acetyl-CoA) synthetase (AceCS) through deacetylation. This conserved regulatory mechanism impacts calorie restriction and lifespan in mammals.

Related Experiment Videos

Last Updated: Jul 18, 2026

Deacetylation Assays to Unravel the Interplay between Sirtuins (SIRT2) and Specific Protein-substrates
14:32

Deacetylation Assays to Unravel the Interplay between Sirtuins (SIRT2) and Specific Protein-substrates

Published on: February 27, 2016

Area of Science:

  • Biochemistry
  • Metabolic Regulation
  • Enzymology

Background:

  • Acetyl-coenzyme A (acetyl-CoA) synthetase (AceCS) is a key enzyme converting acetate to acetyl-CoA.
  • Sirtuins are a class of enzymes known to regulate cellular metabolism through deacetylation.
  • Understanding AceCS regulation is crucial for metabolic research.

Purpose of the Study:

  • To investigate the role of sirtuin-mediated deacetylation in regulating mammalian AceCS activity.
  • To determine if the regulatory mechanism of AceCS is conserved across species.
  • To explore the implications of this regulatory network for calorie restriction and lifespan.

Main Methods:

  • Enzyme activity assays
  • Biochemical analyses
  • Comparative studies of bacterial and mammalian AceCS

Main Results:

  • Sirtuin-mediated deacetylation activates both bacterial and mammalian AceCS.
  • The mechanism of AceCS regulation by sirtuins is conserved between bacteria and mammals.
  • This conserved pathway is critical for controlling acetate metabolism.

Conclusions:

  • Sirtuin-dependent regulation of AceCS represents a conserved metabolic control point.
  • This finding provides insights into the molecular mechanisms underlying calorie restriction.
  • The conserved regulatory network has significant implications for understanding and modulating mammalian lifespan.