Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

On the decrease in lateral mobility of phospholipids by sugars.

Geert van den Bogaart1, Nicolaas Hermans, Victor Krasnikov

  • 1Biochemistry Department, Ultrafast Laser and Spectroscopy Laboratory, Groningen Biomolecular Science and Biotechnology Institute and Zernike Institute for Advanced Materials, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands.

Biophysical Journal
|December 5, 2006
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Catestatin restores cardiac metabolic flexibility and enhances mitochondrial function.

Communications biology·2026
Same author

RNA promotes synapsin phase separation providing a platform for local translation.

bioRxiv : the preprint server for biology·2026
Same author

Condition-dependent amorphous protein agglomerates control cytoplasmic rheology.

Molecular cell·2026
Same author

Autonomous Synthesis and Scrambling of Phospholipids, Linked to Recycling of Cofactors in Synthetic Cells.

ACS synthetic biology·2026
Same author

Structural and functional implications of phase separation of membrane protein LacY in Escherichia coli.

Nature communications·2026
Same author

Membrane-protein-mediated phase separation orchestrates organelle contact sites.

Molecular cell·2026
Same journal

Tau protein differentially affects Piezo1 and Kir2.1 channels in brain capillary endothelial cells.

Biophysical journal·2026
Same journal

Emergent Intercellular Junction Stability during Cyclic Tissue Loading.

Biophysical journal·2026
Same journal

Enhanced-Sampling Simulations Reveal Distinct Intermediates in SARS-CoV-2 FSE Pseudoknot Interconversion.

Biophysical journal·2026
Same journal

Structure-based simulations of the full Flock House virus capsid reveal pathways and energetics of an infection-critical peptide externalization event.

Biophysical journal·2026
Same journal

Quantifying the Peripheral Surface Information Entropy from Conformational Ensembles of Globular Protein-Peptide Complexes.

Biophysical journal·2026
Same journal

Anisotropic unbinding and location-dependent hovering of a kinesin motor head over microtubule.

Biophysical journal·2026
See all related articles

Sucrose and trehalose protect cell membranes during stress. Sucrose more effectively reduces phospholipid mobility in membranes than trehalose, due to stronger interactions, enhancing membrane protection.

Area of Science:

  • Biochemistry
  • Biophysics
  • Membrane Biology

Background:

  • Sugars like sucrose and trehalose are known to protect biological membranes and proteins from damage caused by dehydration and other stresses.
  • Understanding the molecular mechanisms behind this protective effect is crucial for various applications, including food preservation and cryopreservation.

Purpose of the Study:

  • To investigate the differential effects of sucrose and trehalose on the physical properties of phospholipid membranes.
  • To elucidate the molecular interactions between these sugars and membrane phospholipids.

Main Methods:

  • Giant unilamellar vesicles (GUVs) incorporating a fluorescent lipid analog were used to study membrane dynamics.
  • Fluorescence correlation spectroscopy (FCS) was employed to measure the lateral mobility of phospholipids.

Related Experiment Videos

  • Atomistic and coarse-grained molecular dynamics (MD) simulations were performed to analyze sugar-lipid interactions at the molecular level.

    • Main Results:

    • Sucrose significantly decreased the lateral mobility of phospholipids in liquid crystalline membranes more than trehalose.
    • Molecular dynamics simulations revealed that sucrose interacts with a greater number of phospholipid headgroups simultaneously compared to trehalose.
    • This enhanced interaction of sucrose with phospholipids directly correlates with the observed reduction in lateral phospholipid mobility.

    Conclusions:

    • Sucrose exhibits a stronger interaction with phospholipid headgroups than trehalose, leading to a more pronounced decrease in membrane fluidity.
    • This enhanced interaction mechanism contributes to the superior protective effect of sucrose on membrane structures under stress conditions.
    • The findings provide molecular insights into how different sugars confer cryoprotection and osmoprotection to biological membranes.