Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Pulmonary Hypertension: Classification and Pathogenesis01:30

Pulmonary Hypertension: Classification and Pathogenesis

Pulmonary hypertension (PH) is a severe health condition in which the mean pulmonary arterial pressure increases to 25 mmHg or more, even when the body is at rest. This high pressure in the blood vessels that transport blood from the heart to the lungs can cause various symptoms, including shortness of breath, can lead to right heart failure, and significantly affect the overall quality of life.
There are various classifications for PH, each relating to different underlying causes and also...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A Sensitized ENU Mutagenesis Screen for Thrombosis Modifiers Identifies Suppressor Variants in Non-mutagenized Parental Generations Due to Antithrombotic Selective Pressures.

bioRxiv : the preprint server for biology·2026
Same author

The response to kidney injury is epigenetically regulated through the activation of bivalent genes.

American journal of physiology. Renal physiology·2026
Same author

A systems genetics approach identifies roles for proteasome factors in heart development and congenital heart defects.

PLoS genetics·2025
Same author

Centriolar protein PIBF1 is required for craniofacial and forebrain development.

Developmental biology·2025
Same author

Inhibition of Hedgehog signaling does not mitigate polycystic kidney disease severity in a Pkd1 mutant mouse model.

Journal of cell science·2025
Same author

A conditional smoothened (smo) allele on an inbred C57BL/6J genetic background has a hypomorphic smo mutant phenotype.

Developmental biology·2024

Related Experiment Video

Updated: Jul 18, 2026

Light-sheet Fluorescence Microscopy to Capture 4-Dimensional Images of the Effects of Modulating Shear Stress on the Developing Zebrafish Heart
06:51

Light-sheet Fluorescence Microscopy to Capture 4-Dimensional Images of the Effects of Modulating Shear Stress on the Developing Zebrafish Heart

Published on: August 10, 2018

Gata4 is necessary for normal pulmonary lobar development.

Kate G Ackerman1, Jianlong Wang, Liqing Luo

  • 1Division of Genetics, Brigham and Women's Hospital, Harvard Medical School New Research Building 458, 77 Avenue Louis Pasteur, Boston, MA 02115, USA. kackerman@rics.bwh.harvard.edu

American Journal of Respiratory Cell and Molecular Biology
|December 5, 2006
PubMed
Summary

Fog2 mutations cause lung defects. A Fog2-Gata4 interaction, not Gata6, is crucial for normal pulmonary lobar structure and accessory lobe bronchus development in mice.

More Related Videos

Studying Wnt Signaling During Patterning of Conducting Airways
13:00

Studying Wnt Signaling During Patterning of Conducting Airways

Published on: October 16, 2016

A Reverse Genetic Approach to Test Functional Redundancy During Embryogenesis
06:59

A Reverse Genetic Approach to Test Functional Redundancy During Embryogenesis

Published on: August 11, 2010

Related Experiment Videos

Last Updated: Jul 18, 2026

Light-sheet Fluorescence Microscopy to Capture 4-Dimensional Images of the Effects of Modulating Shear Stress on the Developing Zebrafish Heart
06:51

Light-sheet Fluorescence Microscopy to Capture 4-Dimensional Images of the Effects of Modulating Shear Stress on the Developing Zebrafish Heart

Published on: August 10, 2018

Studying Wnt Signaling During Patterning of Conducting Airways
13:00

Studying Wnt Signaling During Patterning of Conducting Airways

Published on: October 16, 2016

A Reverse Genetic Approach to Test Functional Redundancy During Embryogenesis
06:59

A Reverse Genetic Approach to Test Functional Redundancy During Embryogenesis

Published on: August 11, 2010

Area of Science:

  • Developmental Biology
  • Genetics
  • Pulmonary Medicine

Background:

  • Mutations in Fog2 gene in mice lead to pulmonary lobar defects.
  • The role of Gata family cofactors in lung development, specifically accessory lobe bronchus formation, remains unclear.

Purpose of the Study:

  • To investigate whether Gata family cofactors mediate the formation of the accessory lobe bronchus.
  • To determine the specific roles of Gata4 and Gata6 in lung development in conjunction with Fog2.

Main Methods:

  • Evaluation of embryonic mouse lungs with missense mutations affecting FOG-GATA protein interaction.
  • Analysis of lung structure in mice with mutations in Gata6, Gata4, or both.
  • Expression analysis of Gata4 and Fog2 in pulmonary mesenchyme during embryonic development.

Main Results:

  • Mice with Gata6 mutations showed normal lung structure.
  • Mutations in Gata4, or in both Gata4 and Gata6, resulted in structural defects mirroring the Fog2 mutant phenotype.
  • Gata4 and Fog2 are co-expressed in the pulmonary mesenchyme during secondary budding, suggesting a functional interaction.

Conclusions:

  • A Fog2-Gata4 protein interaction is critical for normal pulmonary lobar structure development.
  • The loss of Gata6 interaction does not influence the observed phenotype.
  • Fog2 and Gata4 in the early pulmonary mesenchyme are key players in patterning the secondary bronchus of the accessory lobe.