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Related Experiment Video

Updated: Jul 18, 2026

Generation of a Three-dimensional Full Thickness Skin Equivalent and Automated Wounding

Generation of a Three-dimensional Full Thickness Skin Equivalent and Automated Wounding

Published on: February 26, 2015

Elastin expression in a newly developed full-thickness skin equivalent.

K R Mewes¹, M Raus, A Bernd

¹Phenion GmbH & Co. KG, Düsseldorf, Germany. karsten.mewes@henkel.com

Skin Pharmacology and Physiology

|December 5, 2006

Summary

A new full-thickness skin model successfully generates an in vivo-like elastic system, mimicking dermal elastic fibers. This animal-free model is promising for studying skin aging and environmental impacts on elasticity.

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Cultivating a Three-dimensional Reconstructed Human Epidermis at a Large Scale

Cultivating a Three-dimensional Reconstructed Human Epidermis at a Large Scale

Published on: May 28, 2021

Area of Science:

Biomedical Engineering
Dermatology
Tissue Engineering

Background:

Human skin resilience relies on elastic fibers, primarily composed of fibrillins and elastin.
Understanding and modeling the skin's elastic system is crucial for cosmetic and pharmaceutical research.
In vitro models are needed to study the impact of compounds on skin elasticity.

Purpose of the Study:

To develop and characterize a full-thickness skin model for studying the dermal elastic system.
To analyze elastin expression and fiber formation in this novel in vitro model.
To establish an animal-free system for investigating factors affecting skin elasticity.

Main Methods:

Cultivation of a full-thickness skin model at the air-liquid interface (ALI) for up to 5 weeks.
Immunohistochemistry to detect elastin deposition and fiber development.
Quantitative analysis of elastin mRNA expression over the culture period.

Main Results:

The skin model developed a fully differentiated epidermis and a dermis with extracellular matrix proteins.
Elastin was detected from day 7 of ALI culture, with accumulating elastin-positive fibers.
Elastin mRNA expression peaked around day 10, indicating dynamic matrix remodeling.

Conclusions:

The developed full-thickness skin model generates an in vivo-like elastic system.
This model effectively mimics key aspects of dermal elastic fibers.
The model serves as a valuable animal-free platform for studying skin aging and elasticity changes.