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Updated: Jul 18, 2026

Evaluation of Oxidative Stress in Biological Samples Using the Thiobarbituric Acid Reactive Substances Assay
Published on: May 12, 2020
Oxidative stress as a mechanism of valproic acid-associated hepatotoxicity
Thomas K H Chang1, Frank S Abbott
1Faculty of Pharmaceutical Sciences, The University of British Columbia, Vancouver, British Columbia, Canada. tchang@interchange.ubc.ca
Abstract:
Valproic acid (2-n-propylpentanoic acid; VPA) has several therapeutic indications, but it is used primarily as an anticonvulsant. VPA is a relatively safe drug, but its use is associated with idiosyncratic hepatotoxicity, which in some cases may lead to fatality. The underlying mechanism responsible for the hepatotoxicity is still not well understood, but various hypotheses have been proposed, including oxidative stress. This article discusses the experimental evidence on the effect of VPA on the various indices of oxidative stress and on the potential role of oxidative stress in VPA-associated hepatotoxicity.
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