Related Experiment Videos
Facile cleavage of RNA via phosphodiester linkage fission by Co(III) complex
Y Matsumoto1, K Yonezawa, M Komiyama
1Institute of Materials Science, University of Tsukuba, Ibaraki, Japan.
Nucleic Acids Symposium Series
|January 1, 1990
Abstract:
Adenylyl (3'-5')adenosine (ApA) is effectively cleaved to two adenosine molecules by [Co(trien)(H2O)2]3+ complex (trien: triethylenetetramine). The complex (0.20 M) accelerates the cleavage by 10(5) fold, decreasing half-life of ApA from 4000 years to 9.3 days. The reaction involves general base catalysis by the hydroxide ion bound to the Co(III) ion for the formation of adenosine 2',3'-cyclic phosphate (A greater than p), followed by the prompt cleavage of the intermediate to adenosine.