Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Synthesis of RNA having cap structure.

R Iwase1, M Maeda, T Fujiwara

  • 1Department of Life Chemistry, Tokyo Institute of Technology, Yokohama, Japan.

Nucleic Acids Symposium Series
|January 1, 1990
PubMed
Summary

Chemists synthesized a 43-nucleotide RNA molecule with a cap structure using phosphotriester and phosphoramidite methods. This chemical synthesis enabled the creation of specific RNA fragments for biological applications.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Increased oxidized form of human serum albumin in patients with diabetes mellitus.

Diabetes research and clinical practice·1992
Same author

Successful pancreatojejunal anastomosis for pancreatoduodenectomy.

Surgery, gynecology & obstetrics·1992
Same author

Differential macrophage-mediated cytotoxicity to P388 leukemia cells and its drug-resistant cells examined by a new MTT assay.

Leukemia research·1992
Same author

A cylinder-shaped balloon catheter for the management of dissecting aneurysms in acute stage.

Herz·1992
Same author

Isolation and partial characterization of SSI-like protease inhibitors from Streptomyces.

FEMS microbiology letters·1992
Same author

Induction of cystine transport activity by stress.

Annals of the New York Academy of Sciences·1992

Area of Science:

  • Molecular Biology
  • Organic Chemistry
  • Biochemistry

Background:

  • Chemical synthesis of RNA is crucial for studying RNA function and developing RNA-based therapeutics.
  • Existing methods for synthesizing long, capped RNA molecules can be complex and inefficient.

Purpose of the Study:

  • To develop a robust chemical synthesis strategy for a 43-nucleotide capped RNA molecule.
  • To demonstrate the feasibility of combining different chemical synthesis approaches for RNA construction.

Main Methods:

  • Synthesis of a 9-nucleotide leader sequence with a cap structure using the phosphotriester method.
  • Chemical synthesis of a 32-nucleotide cistron fragment using the phosphoramidite method.
  • Ligation of RNA fragments and introduction of a modified 3'-end nucleotide using T4 RNA ligase.

Related Experiment Videos

Main Results:

  • Successful synthesis of a 43-nucleotide RNA molecule containing a cap structure.
  • Demonstrated efficient ligation of chemically synthesized RNA fragments.
  • Incorporation of a modified guanosine 5'-phosphate at the 3'-end of the RNA.

Conclusions:

  • The study presents a viable chemical synthesis approach for producing capped RNA molecules of significant length.
  • This method provides a foundation for generating custom RNA constructs for research and therapeutic purposes.