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Related Experiment Video

Updated: Jul 18, 2026

In Vitro Polymerization of F-actin on Early Endosomes

In Vitro Polymerization of F-actin on Early Endosomes

Published on: August 28, 2017

New component of ESCRT-I regulates endosomal sorting complex assembly.

Tony Chu¹, Ji Sun, Suraj Saksena

¹Department of Cellular and Molecular Medicine, University of California, San Diego, La Jolla, CA 92093, USA.

The Journal of Cell Biology

|December 6, 2006

Summary

The study identifies Mvb12 as a new ESCRT-I component that stabilizes the complex in an inactive state. This ensures proper ESCRT machinery assembly and function at the endosome for multivesicular body sorting.

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Area of Science:

Cell Biology
Molecular Mechanisms
Protein Complexes

Background:

Endosomal Sorting Complex Required for Transport (ESCRT) complexes are crucial for receptor down-regulation and viral budding.
The precise assembly and regulation mechanisms of ESCRT machinery remain incompletely understood.
Existing structural data for ESCRT complexes do not fully elucidate their dynamic functions.

Purpose of the Study:

To identify and characterize novel components of the ESCRT-I complex.
To investigate the role of new factors in ESCRT-I assembly and regulation.
To elucidate the molecular basis for spatial and temporal control of ESCRT function in endosomal sorting.

Main Methods:

Biochemical identification of a new ESCRT-I component, Mvb12.
Co-immunoprecipitation assays to study protein interactions.
Analysis of ESCRT complex assembly and oligomerization in vitro and in vivo.

Main Results:

Mvb12 was identified as a novel ESCRT-I component that binds to the Vps23 subunit.
Mvb12 and the Vps23 coiled-coil domain are essential for ESCRT-I oligomerization in the cytosol.
Loss of Mvb12 leads to ESCRT-I disassembly and stable ESCRT-I/-II complex formation in the cytosol.

Conclusions:

Mvb12 acts as a key regulator, stabilizing ESCRT-I in an inactive, oligomeric state in the cytosol.
This stabilization ensures the timely and localized recruitment of ESCRT-I and -II to the endosome.
The findings provide insights into the ordered assembly of ESCRT machinery for multivesicular body sorting.