Related Experiment Video
Updated: Jul 18, 2026
Hydrodynamic Renal Pelvis Injection for Non-viral Expression of Proteins in the Kidney
Published on: January 8, 2018
[Construction and expression of ATP50 fluorescent protein in TM3 mouse Leydig cells]
Liang Chen1, Zhong-cheng Xin, Xue-jun Jiang
1Andrology Center, First Hospital, Peking University, Beijing 100009, China. bdyychenliang@163.com
Objective:
To study the expression and localization of ATP50 by construction of ATP50-pEYFP-N1 in primary cultured mouse Leydig cells.
Methods:
Primary cultured mouse Leydig cells were confirmed by 3B-HSD staining. ATP50 was cloned into pEYFP-N1 between Bam HI and Eco RI sites. Cell-transfection and living-cell fluorescence imaging microscopy were employed to investigate the sub-cellular localization of YFP-ATP50 in TM3 mouse Leydig cells.
Results:
ATP50 green fluorescent protein was well co-localized with red fluorescence mitochondrion marker-Mitotracker in TM3 mouse Leydig cells.
Conclusion:
ATP50 was expressed in primary cultured mouse Leydig cells. The fluorescent expression vector of ATP50 was constructed successfully and YFP-ATP50 was located in mitochondria in TM3 mouse Leydig cells, which provided a useful clue for further research on the steroidogenesis dysfunction in aging males.
