[Preparation and identification of anti-human epididymal sperm protein P34H monoclonal antibody]
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Updated: Jul 18, 2026
Analysis of Epididymal Protein Synthesis and Secretion
Published on: August 25, 2018
Xin-yi Xia1, Yong-ming Wu, Lian-jun Pan
1Laboratory of Reproduction & Genetics, School of Clinical Medicine, Nanjing University Medical College, Nanjing General Hospital of Nanjing Command, PLA, Nanjing, Jiangsu 210002, China. xiaxy@androl.cn
Objective:
To prepare and identify anti-human epididymal sperm protein P34H monoclonal antibody (McAb).
Methods:
The previously purified recombinant P34H protein was used as an antigen to immunize BALB/c mouse. Cell strains secreting anti-P34H McAb were established by hybridoma technique and then ascitic fluid-type McAb prepared. The sensitivity and specificity of McAb were detected by ELISA and Western blot, respectively.
Results:
One strain (2C4) of IgG1 Kappa anti-P34H McAb was harvested. The titer detected by ELISA technique was 1:10(3) - 1:10(5). Western blot of healthy human sperm samples and purified recombinant P34H antigen probed with the prepared McAb were immunoreactive at 34,000 and 27,000, respectively.
Conclusion:
Anti-P34H McAb has been prepared successfully by the above methods, which may provide a powerful tool for exploring the relationship between P34H and male fertility.
