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Updated: Jul 18, 2026
Quantifying the Modulation of Elastase Enzyme Activity Through Colorimetric Analysis
Published on: January 17, 2025
Inhibition of elastase activity by essential oils in vitro
Masahiro Mori1, Norikazu Ikeda, Yoshiko Kato
1Research and Development Division, Institute of Cosmetic Science, Club Cosmetics Co. Ltd, Japan. moori@clubcosmetics.co.jp
Background:
Essential oils are widely used, for example in aromatherapy and aroma massage. In aroma massage, essential oil, diluted with vegetable oil, is rubbed onto the skin. Components of essential oil penetrate into the skin and have an influence on the dermis. Elastase is an enzyme which degenerates dermal elastin. Elastase activity is believed to contribute to cutaneous wrinkling and ageing.
Aim:
To investigate the inhibitory effect of essential oils on elastase activity.
Methods:
Inhibition of elastase activity by various essential oils was assessed using two elastase enzymes: porcine pancreatic elastase (PPE) and human neutrophil elastase (HNE).
Results:
Elastase activity was inhibited by various essential oils, especially by those oils derived from lemons, juniper and grapefruit. Although the specific inhibitory component was not determined, lemon oil had the greatest inhibitory effect on PPE. Some essential oils also inhibited HNE.
Conclusions:
These studies demonstrate a possible rationale for the use of essential oil massage as a preventive treatment for cutaneous wrinkling and ageing.
