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Acid ethanol fixation and polyester wax embedding combines preservation of antigenic determinants with good

P J Roholl1, H F Dullens, J Kleijne

  • 1IVEG-TNO, Rijswijk, The Netherlands.

Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission
|January 1, 1991
PubMed

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Fixation and Sectioning01:03

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Two basic types of preparation are used to visualize specimens with a light microscope: wet mounts and fixed specimens.
The simplest type of preparation is the wet mount, in which the specimen is placed in a drop of liquid on the slide. A liquid specimen can be directly deposited on the slide using a dropper. Solid specimens, such as skin scraping, can be placed on the slide before adding a drop of liquid to prepare the wet mount. Sometimes the liquid is simply water, but stains are often added...