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Related Experiment Video

Updated: Jul 18, 2026

Assessment of Acute Wound Healing using the Dorsal Subcutaneous Polyvinyl Alcohol Sponge Implantation and Excisional Tail Skin Wound Models.

Assessment of Acute Wound Healing using the Dorsal Subcutaneous Polyvinyl Alcohol Sponge Implantation and Excisional Tail Skin Wound Models.

Published on: March 25, 2020

Interleukin-1alpha gene expression during wound healing.

F M Robertson¹, A E Pellegrini, M S Ross

¹The Ohio State University Comprehensive Cancer Center, Columbus, Ohio 43210, USA.

Wound Repair and Regeneration : Official Publication of the Wound Healing Society [And] the European Tissue Repair Society

|October 1, 1995

Summary

Interleukin-1alpha (IL-1α) gene expression rapidly increases after wounding, with IL-1α protein found in keratinocytes and neutrophils. This suggests IL-1α is crucial for initiating early wound healing cellular events.

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Assessment of Acute Wound Healing using the Dorsal Subcutaneous Polyvinyl Alcohol Sponge Implantation and Excisional Tail Skin Wound Models.

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Published on: September 29, 2021

Area of Science:

Wound healing research
Immunology
Cell biology

Background:

Interleukin-1alpha (IL-1α) is constitutively produced by keratinocytes and enhances wound reepithelialization.
Previous studies lacked temporal characterization of IL-1α gene expression and identification of its cellular sources during wound healing.

Purpose of the Study:

To characterize the temporal sequence of IL-1α gene expression during wound healing.
To identify the cellular sources of IL-1α protein in wound tissue.

Main Methods:

Quantification of IL-1α messenger RNA (mRNA) levels in wound tissue from SKH-1 hairless mice over a 10-day period.
Immunohistochemical analysis to identify cells expressing IL-1α immunoreactive protein.

Main Results:

A time-dependent, four-fold increase in IL-1α gene expression was observed within 4 hours post-wounding.
Increased IL-1α levels correlated with neutrophil infiltration and proliferation of keratinocytes and dermal fibroblasts.
IL-1α protein was localized to keratinocytes at the wound edge/epidermis and neutrophils in the dermis.

Conclusions:

IL-1α plays a significant role in the early stages of wound healing.
Early IL-1α upregulation suggests local and systemic actions initiating critical cellular events, including keratinocyte proliferation and migration, and leukocyte chemotaxis.