Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Phases of Wound Repair

Phases of Wound Repair

Following injury, the integrity of the injured tissues must be reestablished. For example, in skin tissue, wound repair involves coordination among resident skin cells, blood mononuclear cells, extracellular matrix, growth factors, and cytokines to complete the healing cascade.
Formation of Blood Clot
In case of deep injuries, trauma to blood vessels results in blood loss. In the meantime, phospholipids released from the ruptured endothelial cellular membrane are converted into arachidonic...

Regulation of Angiogenesis and Blood Supply

Regulation of Angiogenesis and Blood Supply

Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits. Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl hydroxylase and factor...

Healing II: Complications

Healing II: Complications

Complications during healing arise when tissue repair is altered by local or systemic factors. These changes involve abnormal collagen deposition, altered biomechanics, and reduced vascular supply, impairing restoration of normal structure and function.Loss of FunctionScar tissue differs significantly from the original tissue it replaces. In the skin, fibrosis lacks adnexal structures such as hair follicles, sebaceous glands, and sweat glands. Their absence reduces tactile sensitivity, impairs...

Clinical Applications of Epidermal Stem Cells

Clinical Applications of Epidermal Stem Cells

Epidermal stem cells (EpiSCs) are mainly located at the basal layer of the epidermis. These cells repair minor injuries of the skin and replace dead skin cells. However, EpiSCs’ cannot heal severe wounds such as major burns or those from diabetes or hereditary disorders. In such cases, culturing the epidermal stem cells from the patient is possible and has yielded successful treatment options, such as laboratory-grown skin grafts. These grafts are synthesized using a patient’s own EpiSCs...

Renewal of Skin Epidermal Stem Cells

Renewal of Skin Epidermal Stem Cells

The skin is divided into epidermis, dermis, and hypodermis, the skin's outermost, middle, and inner layers. The human epidermal layer regularly undergoes renewal, where old, dead cells are replaced by new cells. Epidermal stem cells or EpiSCs divide and differentiate to restore the lost cells. For the renewal process, some EpiSCs continuously self-renew. In contrast, few others differentiate into transit-amplifying cells, which later form prickle or spinous cells, followed by granular cells,...

Treatment for Pulmonary Arterial Hypertension: Endothelin Receptor Antagonists

Treatment for Pulmonary Arterial Hypertension: Endothelin Receptor Antagonists

Endothelins (ETs) are potent vasoactive peptides critical in the human body's various physiological and pathological processes. One of the most promising therapeutic strategies for treating pulmonary arterial hypertension (PAH) involves counteracting the effects of these endothelins using a class of drugs known as endothelin receptor antagonists.
ETs are synthesized through a complex sequence of enzymatic steps, primarily involving an enzyme referred to as endothelin-converting enzyme (ECE). Of...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

[Update in lung transplantation].

Zeitschrift fur Herz-, Thorax- und Gefasschirurgie·2020

Same author

Role of Adult Tissue-Derived Pluripotent Stem Cells in Bone Regeneration.

Stem cell reviews and reports·2019

Same author

Electrical stimulation shifts healing/scarring towards regeneration in a rat limb amputation model.

Scientific reports·2019

Same author

Changes in serum KL-6 levels are associated with the development of chronic lung allograft dysfunction in lung transplant recipients.

Transplant immunology·2018

Same author

Necrotizing fasciitis: treatment concepts and clinical results.

European journal of trauma and emergency surgery : official publication of the European Trauma Society·2017

Same author

Frankfurt microsurgery course: the first 175 trainees.

European journal of trauma and emergency surgery : official publication of the European Trauma Society·2017

Search research articles

Related Experiment Video

Updated: Jul 18, 2026

A Novel In Vitro Wound Healing Assay to Evaluate Cell Migration

A Novel In Vitro Wound Healing Assay to Evaluate Cell Migration

Published on: March 17, 2018

Ketanserin accelerates wound epithelialization and neovascularization.

M Kim¹, E T Ustüner, D Schuschke

¹Division of Plastic and Reconstructive Surgery and Center for Microcirculatory Studies, University of Louisville, KY 40292, USA.

Wound Repair and Regeneration : Official Publication of the Wound Healing Society [And] the European Tissue Repair Society

|October 1, 1995

Summary

Topical ketanserin, a serotonin receptor blocker, significantly accelerates wound healing in mice. This study found enhanced vascularization and epithelialization, suggesting potential for improved skin wound treatment.

More Related Videos

Digital Planimetry for Assessing Wound Closure Kinetics in a Mouse Model

Digital Planimetry for Assessing Wound Closure Kinetics in a Mouse Model

Published on: January 10, 2025

An Epithelial Abrasion Model for Studying Corneal Wound Healing

An Epithelial Abrasion Model for Studying Corneal Wound Healing

Published on: December 29, 2021

Related Experiment Videos

Last Updated: Jul 18, 2026

A Novel In Vitro Wound Healing Assay to Evaluate Cell Migration

A Novel In Vitro Wound Healing Assay to Evaluate Cell Migration

Published on: March 17, 2018

Digital Planimetry for Assessing Wound Closure Kinetics in a Mouse Model

Digital Planimetry for Assessing Wound Closure Kinetics in a Mouse Model

Published on: January 10, 2025

An Epithelial Abrasion Model for Studying Corneal Wound Healing

An Epithelial Abrasion Model for Studying Corneal Wound Healing

Published on: December 29, 2021

Area of Science:

Dermatology
Pharmacology
Wound Healing Research

Background:

Wound healing involves complex processes including vascularization and epithelialization.
Serotonin receptors (5-HT2) play a role in physiological processes that may influence wound repair.
Topical agents are explored for their potential to modulate and enhance wound healing outcomes.

Purpose of the Study:

To investigate the acute effects of topical ketanserin on wound epithelialization and vascularization.
To determine the efficacy of different concentrations of ketanserin in promoting skin wound healing.
To assess the impact of ketanserin on arteriolar diameters in the context of wound repair.

Main Methods:

Utilized the hairless mouse ear model for standardized full-thickness skin wounds.
Administered varying concentrations of ketanserin (0%, 0.2%, 2.0%, 20% w/v) post-surgery and daily.
Employed video microscopy and computer-assisted planimetry to track vascularization and epithelialization.
Measured arteriole diameters before and after wounding.

Main Results:

Topical ketanserin significantly accelerated vascularization rates (p < 0.001 at 2% and 20% concentrations).
Significant acceleration of epithelialization was observed at a 20% concentration (p < 0.001).
Vasodilation of terminal arterioles was not identified as a primary mechanism of action.

Conclusions:

Topically applied ketanserin effectively enhances both vascular and epithelial components of wound healing in full-thickness skin wounds.
The accelerated epithelialization may be attributed to a direct effect of ketanserin on epithelial cells.
Ketanserin demonstrates potential as a therapeutic agent for improving skin wound healing rates.