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Related Experiment Videos

Two methods for flat embedding sections in LR white cut from paraffin blocks.

A Migheli1, A Attanasio

  • 12nd Neurologic Clinic, University of Turin, Italy.

Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission
|January 1, 1991
PubMed
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Two simple methods embed brain tissue sections in LR White resin by exploiting its ability to polymerize. These techniques allow for the collection of large tissue areas for immunocytochemistry.

Area of Science:

  • Neuroscience
  • Histology
  • Biochemistry

Background:

  • Paraffin embedding is a common method for preserving tissue structure.
  • Embedding tissue in resin is crucial for advanced microscopy and immunocytochemistry.
  • LR White resin offers good hydrophilicity and compatibility with biological samples.

Purpose of the Study:

  • To describe two novel, simple methods for flat embedding of brain tissue sections.
  • To optimize the embedding process for subsequent immunocytochemical analysis.
  • To facilitate the collection of large, intact tissue areas for detailed study.

Main Methods:

  • Exploiting the polymerization capability of fresh LR White resin when added to cured resin.
  • Method 1: Pressing a tissue section in LR White into a gelatin capsule using a blank block.

Related Experiment Videos

  • Method 2: Sandwiching a tissue section in LR White between the halves of a sawn blank block.

    • Main Results:

    • Successful flat embedding of brain tissue sections in LR White resin using both described methods.
    • Demonstrated the ability to collect thin sections containing large areas of tissue after curing.
    • Prepared sections are suitable for further processing in immunocytochemistry.

    Conclusions:

    • The described methods provide a simple and effective approach for embedding brain tissue in LR White resin.
    • These techniques enhance the ability to obtain large tissue sections for immunocytochemistry.
    • The methods are valuable for researchers in neuroscience and histology requiring high-quality tissue preservation.