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Related Concept Videos

Formation of Muscle Fibers from Myoblasts01:13

Formation of Muscle Fibers from Myoblasts

De novo myogenesis, or the formation of muscle fibers, begins during the early embryonic stages. The skeletal muscle is formed from somites– blocks of embryonic cell layers. The somites are further divided into dermatomes, myotomes, sclerotomes, and syndetomes. Among these, the myotomes give rise to muscle fibers.
Muscle progenitor cells (MPCs) are formed from the myotomes. MPCs express genes that encode the transcription factors Pax3 and Pax7. Along with Pax 3/7, other transcription factors...
Satellite Stem Cells and Muscular Dystrophy01:21

Satellite Stem Cells and Muscular Dystrophy

Satellite stem cells or myosatellite cells are quiescent stem cells that Alexander Mauro first identified in 1961. These cells are located between the sarcolemma, the plasma membrane of muscle fibers, and the basal lamina, the connective tissue sheath covering it. These mononucleated cells are activated in response to muscle injury, can transform into myoblasts, and may form or repair muscle fibers. Myosatellite cells can provide additional myonuclei for muscle regeneration or return to a...
In-vitro Mutagenesis01:16

In-vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.

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Related Experiment Video

Updated: Jul 18, 2026

Preparation of Primary Myogenic Precursor Cell/Myoblast Cultures from Basal Vertebrate Lineages
07:51

Preparation of Primary Myogenic Precursor Cell/Myoblast Cultures from Basal Vertebrate Lineages

Published on: April 30, 2014

Muscle growth after postdevelopmental myostatin gene knockout.

Stephen Welle1, Kirti Bhatt, Carl A Pinkert

  • 1Department of Medicine, University of Rochester, Rochester, NY, USA. stephen_welle@urmc.rochester.edu

American Journal of Physiology. Endocrinology and Metabolism
|December 7, 2006
PubMed
Summary

Knocking out the myostatin gene in adult mice significantly increases muscle mass by promoting fiber hypertrophy. This finding demonstrates myostatin

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Stable Knockdown of Genes Encoding Extracellular Matrix Proteins in the C2C12 Myoblast Cell Line Using Small-Hairpin (sh)RNA
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Transplantation of Induced Pluripotent Stem Cell-derived Mesoangioblast-like Myogenic Progenitors in Mouse Models of Muscle Regeneration
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Transplantation of Induced Pluripotent Stem Cell-derived Mesoangioblast-like Myogenic Progenitors in Mouse Models of Muscle Regeneration

Published on: January 20, 2014

Related Experiment Videos

Last Updated: Jul 18, 2026

Preparation of Primary Myogenic Precursor Cell/Myoblast Cultures from Basal Vertebrate Lineages
07:51

Preparation of Primary Myogenic Precursor Cell/Myoblast Cultures from Basal Vertebrate Lineages

Published on: April 30, 2014

Stable Knockdown of Genes Encoding Extracellular Matrix Proteins in the C2C12 Myoblast Cell Line Using Small-Hairpin (sh)RNA
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Stable Knockdown of Genes Encoding Extracellular Matrix Proteins in the C2C12 Myoblast Cell Line Using Small-Hairpin (sh)RNA

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Transplantation of Induced Pluripotent Stem Cell-derived Mesoangioblast-like Myogenic Progenitors in Mouse Models of Muscle Regeneration
10:03

Transplantation of Induced Pluripotent Stem Cell-derived Mesoangioblast-like Myogenic Progenitors in Mouse Models of Muscle Regeneration

Published on: January 20, 2014

Area of Science:

  • Muscle physiology
  • Molecular biology
  • Genetics

Background:

  • Myostatin is a key regulator of muscle mass.
  • Constitutive myostatin gene knockout causes excessive muscle growth during development.

Purpose of the Study:

  • To investigate the effect of myostatin gene knockout on mature muscle.
  • To determine if myostatin inhibition can induce muscle growth after development.

Main Methods:

  • Generated mice with floxed myostatin exon 3 (Mstn[f/f]).
  • Utilized tamoxifen-inducible Cre recombinase for conditional knockout.
  • Administered tamoxifen to 4-month-old Mstn[f/f]/Cre+ mice to induce knockout.

Main Results:

  • Postdevelopmental myostatin knockout reduced myostatin mRNA to <1% of normal.
  • Muscle mass increased by approximately 25% in knockout mice over 3 months.
  • Fiber hypertrophy, not altered myosin heavy-chain isoform expression, explained the muscle mass increase.

Conclusions:

  • Postdevelopmental myostatin gene knockout significantly increases muscle mass.
  • Myostatin inhibition is effective in inducing muscle growth even after development has ceased.
  • This suggests therapeutic potential for conditions involving muscle loss.