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Induction of apoptosis and histone hyperacetylation by diallyl disulfide in prostate cancer cell line PC-3

Arumugam Arunkumar1, Marati Radhakrishnan Vijayababu, Nandagopal Gunadharini

  • 1Department of Endocrinology, Dr. ALM PG Institute of Basic Medical Sciences, University of Madras, Taramani, Chennai, Tamil Nadu 600 113, India.

Cancer Letters
|December 8, 2006
PubMed

Insights

Diallyl disulfide (DADS), a compound found in garlic, effectively induces apoptosis, or programmed cell death, in prostate cancer cells. This occurs through a mechanism involving histone hyperacetylation, offering a potential new strategy for cancer treatment.

Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • Prostate cancer progresses from androgen-dependent to androgen-independent stages.
  • Apoptosis induction is a key strategy for managing advanced prostate cancer.
  • Histone acetylation regulates gene expression, influencing cell proliferation and apoptosis.

Purpose of the Study:

  • To investigate the effects of Diallyl disulfide (DADS) on histone acetylation and apoptosis in prostate cancer cells.
  • To explore DADS as a potential therapeutic agent for androgen-independent prostate cancer.

Main Methods:

  • In vitro study using prostate cancer cell lines.
  • Annexin V-FITC binding assay to detect apoptosis.
  • Caspase-3 activity assay to quantify apoptosis.
  • Assessment of histone acetylation levels.

Main Results:

  • DADS treatment dose-dependently induced apoptosis in prostate cancer cells.
  • Increased numbers of early and late apoptotic cells were observed at 25 and 40 microM DADS.
  • DADS treatment led to significant histone hyperacetylation in treated cells.

Conclusions:

  • Diallyl disulfide (DADS) effectively induces apoptosis in prostate cancer cells.
  • DADS exerts its pro-apoptotic effects by influencing histone acetylation.
  • DADS represents a promising therapeutic candidate for prostate cancer treatment.

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