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Related Concept Videos

DNA Base Pairing02:27

DNA Base Pairing

Erwin Chargaff’s rules on DNA equivalence paved the way for the discovery of base pairing in DNA. Chargaff’s rules state that in a double-stranded DNA molecule,
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Transfer RNA Synthesis02:36

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Related Experiment Video

Updated: Jul 18, 2026

A Study of the Complexation of Mercury(II) with Dicysteinyl Tetrapeptides by Electrospray Ionization Mass Spectrometry
12:59

A Study of the Complexation of Mercury(II) with Dicysteinyl Tetrapeptides by Electrospray Ionization Mass Spectrometry

Published on: January 8, 2016

Structural analyses on the mercuryII-mediated T-T base pair.

Yoshiyuki Tanaka1, Shuji Oda, Hiroshi Yamaguchi

  • 1Graduate School of Pharmaceutical Sciences, Tohoku University, Aobayama, Aoba-ku, Sendai 980-8578, Japan.

Nucleic Acids Symposium Series (2004)
|December 8, 2006
PubMed
Summary

This study reveals how mercury(II) ions bind to DNA. Mercury(II) specifically binds to T-T mismatches, forming T-Hg(II)-T pairs and causing structural changes in the DNA duplex.

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Last Updated: Jul 18, 2026

A Study of the Complexation of Mercury(II) with Dicysteinyl Tetrapeptides by Electrospray Ionization Mass Spectrometry
12:59

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Published on: January 8, 2016

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Published on: June 8, 2022

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Chemical Biology

Background:

  • T-T mismatches in DNA can specifically recognize mercury(II) ions.
  • The formation of T-Hg(II)-T pairs has been previously reported.

Purpose of the Study:

  • To investigate the structure and properties of T-Hg(II)-T pairs.
  • To understand the binding mechanism of mercury(II) to DNA duplexes with T-T mismatches.

Main Methods:

  • NMR spectroscopy was used to study a DNA duplex containing two successive T-T mismatches.
  • 1D proton (1H) NMR titration experiments with mercury(II) were performed.

Main Results:

  • Imino proton signals of T-T mismatches disappeared upon mercury(II) addition.
  • Additional signals of transient mono-mercurated duplexes were observed, indicating slow structural transformations.
  • Evidence suggests mercury(II) cross-links two thymine bases via their N3 atoms, forming T-Hg(II)-T pairs.

Conclusions:

  • The study provides structural insights into mercury(II) binding to DNA.
  • The findings confirm the formation of T-Hg(II)-T pairs and highlight the dynamic nature of mercury(II)-DNA interactions.