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A Protocol for Computer-Based Protein Structure and Function Prediction
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AllerTool: a web server for predicting allergenicity and allergic cross-reactivity in proteins.

Zong Hong Zhang1, Judice L Y Koh, Guang Lan Zhang

  • 1Institute for Infocomm Research, 21 Heng Mui Keng Terrace, Singapore 119613. zhzhang@i2r.a-star.edu.sg

Bioinformatics (Oxford, England)
|December 8, 2006
PubMed
Summary
This summary is machine-generated.

Introducing AllerTool, a new web server for assessing potential allergenicity and cross-reactivity patterns of food proteins. This tool aids in evaluating known and predicted cross-reactivity, crucial for food safety and novel protein introduction.

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Area of Science:

  • Bioinformatics
  • Allergology
  • Food Safety

Background:

  • Assessing potential allergenicity and cross-reactivity is vital for novel proteins in the human food chain.
  • Current bioinformatics tools primarily predict allergenicity, lacking cross-reactivity pattern information.
  • AllerTool addresses this gap by providing tools for both predicted and published cross-reactivity patterns.

Purpose of the Study:

  • To introduce AllerTool, a web server designed for assessing allergen cross-reactivity.
  • To provide tools for evaluating predicted and known cross-reactivity patterns of allergens.
  • To support the assessment of novel proteins introduced into the human food chain.

Main Methods:

  • Development of AllerTool, a web server with integrated analysis tools.
  • Inclusion of graphical representation of allergen cross-reactivity data.
  • Implementation of local sequence comparison and sequence similarity search tools.
  • Utilization of a support vector machine (SVM) method for cross-reactivity assessment.

Main Results:

  • The SVM model achieved an area under the receiver operating curve (A(ROC)) of 0.90.
  • The model demonstrated 86.00% sensitivity (SE) and 86.00% specificity (SP) in 10-fold cross-validation.
  • AllerTool provides comprehensive tools for allergen cross-reactivity analysis.

Conclusions:

  • AllerTool offers essential functionalities for assessing allergen cross-reactivity patterns.
  • The tool aids in evaluating potential allergenicity and cross-reactivity in accordance with FAO/WHO Codex alimentarius guidelines.
  • AllerTool is a valuable resource for researchers and regulatory bodies involved in food safety and allergen management.