Related Experiment Video
Updated: Jun 30, 2026
The Multifaceted Benefits of Protein Co-expression in Escherichia coli
Published on: February 5, 2015
A novel high-copy plasmid, pEC, compatible with commonly used Escherichia coli cloning and expression vectors
Huijing Zhang1, Haizhen Wu, Huizhan Zhang
1State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, China.
Abstract:
A 66164-bp cryptic plasmid, pEIB1, was isolated from strain Vibrio anguillarum MVM425 and sequenced. A plasmid carrying a 1089-bp fragment, containing the minimal replication region of pEIB1, a kanamycin-resistance marker and an L-arabinose promoter, designated pEC, was maintained as a high copy plasmid in E. coli and stably inherited in the absence of antibiotic selection. Significantly, pEC was compatible with the widely used ColE1, pSC101 and p15A replicons making it a useful tool for a dual-plasmid expression system.
