Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

In-cell protein dynamics.

Julie E Bryant1

  • 1Department of Chemistry, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA. bryant.je@gmail.com

Molecular Biosystems
|December 13, 2006
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Cytosol has a small effect on protein backbone dynamics.

Biochemistry·2006
Same author

Protein dynamics in living cells.

Biochemistry·2005
Same journal

Imbalance in amino acid and purine metabolisms at the hypothalamus in inflammation-associated depression by GC-MS.

Molecular bioSystems·2017
Same journal

Correction: Dynamic properties of dipeptidyl peptidase III from Bacteroides thetaiotaomicron and the structural basis for its substrate specificity - a computational study.

Molecular bioSystems·2017
Same journal

Conformational heterogeneity in tails of DNA-binding proteins is augmented by proline containing repeats.

Molecular bioSystems·2017
Same journal

Mechanism of the formation of the RecA-ssDNA nucleoprotein filament structure: a coarse-grained approach.

Molecular bioSystems·2017
Same journal

Staphylococcus aureus extracellular vesicles (EVs): surface-binding antagonists of biofilm formation.

Molecular bioSystems·2017
Same journal

Development of an AlphaLISA high throughput technique to screen for small molecule inhibitors targeting protein arginine methyltransferases.

Molecular bioSystems·2017
See all related articles

The intracellular environment of proteins in E. coli does not change protein structure or fast dynamics. However, cytosol viscosity slightly increases the timescale of these motions.

Area of Science:

  • Biophysics
  • Molecular Biology
  • Protein Dynamics

Background:

  • Cellular environments are crowded, unlike dilute solutions used for most protein studies.
  • Understanding protein behavior in vivo is crucial for biological function.

Purpose of the Study:

  • To investigate if the native intracellular environment affects protein dynamics compared to dilute solutions.
  • To compare the fast backbone dynamics of apocytochrome b5 in living E. coli versus dilute solution.

Main Methods:

  • Utilized nuclear magnetic resonance (NMR) spectroscopy to measure 15N relaxation.
  • Studied uniformly 15N-enriched apocytochrome b5 within living Escherichia coli.
  • Employed Lipari-Szabo analysis to compare protein dynamics in cellular versus solution states.

Related Experiment Videos

Main Results:

  • The intracellular environment of E. coli did not alter the overall structure of apocytochrome b5.
  • Fast backbone dynamics (amplitude) remained largely unchanged in the cellular environment.
  • The average timescale of fast backbone motions was increased in the cytosol, likely due to increased viscosity.

Conclusions:

  • The native cellular environment minimally impacts protein structure and fast dynamics.
  • Cytosolic viscosity is a key factor influencing the timescale of protein motions in vivo.
  • NMR spectroscopy is effective for studying protein dynamics within living cells.