Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Long-range cooperative interactions modulate dimerization in SARS 3CLpro.

Jennifer Barrila1, Usman Bacha, Ernesto Freire

  • 1Department of Biology, Johns Hopkins University, Baltimore, Maryland 21218, USA.

Biochemistry
|December 13, 2006
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Cross-cohort meta-analysis reveals conserved gut microbiome signatures of insomnia.

Current research in microbial sciences·2026
Same author

Microgravity-induced constraints on melanin bioproduction: investigating E. coli metabolic responses aboard the international space station.

NPJ microgravity·2026
Same author

Navigating mental health in space: gut-brain axis and microbiome dynamics.

Experimental & molecular medicine·2025
Same author

Microbiology of human spaceflight: microbial responses to mechanical forces that impact health and habitat sustainability.

Microbiology and molecular biology reviews : MMBR·2024
Same author

Incremental increases in physiological fluid shear progressively alter pathogenic phenotypes and gene expression in multidrug resistant <i>Salmonella</i>.

Gut microbes·2024
Same author

Extrapolating differential scanning calorimetry data for monoclonal antibodies to low temperatures.

Analytical biochemistry·2024
Same journal

Aromatic Cage-Directed Azide-Methyllysine Photochemistry for Profiling Nonhistone Interacting Partners of the MeCP2 Methyl-CpG-Binding Domain.

Biochemistry·2026
Same journal

Differential Hydroxypyruvate Processing by <i>E. coli</i> and <i>P. aeruginosa</i> DXP Synthases Reveals Preferential Xylulose 5-Phosphate Formation by the <i>P. aeruginosa</i> Enzyme.

Biochemistry·2026
Same journal

Structural and Functional Characterization of Heterologous Nitrogenase Complexes.

Biochemistry·2026
Same journal

Discovery of Bacterial Unspecific Peroxygenases.

Biochemistry·2026
Same journal

Lactate Biology: Subcellular Routing and Chemical Form Define Function.

Biochemistry·2026
Same journal

Nature's Anaerobic Toolkit: Glycyl Radical Enzymes and Their Expanding Functional and Mechanistic Diversity.

Biochemistry·2026
See all related articles

Researchers discovered that targeting serine residues away from the active site can inhibit SARS-CoV dimerization. This finding offers new strategies for developing antiviral therapies against SARS-CoV 3CLpro.

Area of Science:

  • Biochemistry
  • Virology
  • Drug Discovery

Background:

  • Severe acute respiratory syndrome (SARS) is caused by SARS-CoV.
  • The SARS main viral protease (SARS 3CLpro) is a key target for antiviral drug development.
  • SARS 3CLpro functions as an inactive homodimer, requiring dimerization for activity.

Purpose of the Study:

  • To investigate allosteric control of SARS 3CLpro dimerization.
  • To identify novel target sites for dimerization inhibitors beyond the active site.

Main Methods:

  • Site-directed mutagenesis of conserved serine residues (Ser139, Ser144, Ser147) adjacent to the active site.
  • Assessing the impact of mutations on SARS 3CLpro catalytic activity and dimerization.

Main Results:

Related Experiment Videos

  • Mutations of serine residues adjacent to the active site significantly impaired protease activity.
  • Mutation of Ser147, located away from the dimer interface, completely inhibited dimerization and abolished enzymatic activity.

Conclusions:

  • SARS 3CLpro dimerization is under allosteric control.
  • Residues distant from the dimer interface can regulate subunit association.
  • These findings define new allosteric targets for designing SARS-CoV dimerization inhibitors.