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Agrobacterium-Mediated Genetic Transformation, Transgenic Production, and Its Application for the Study of Male Reproductive Development in Rice
Published on: October 6, 2020
Transgenic indica rice resistant to sap-sucking insects
D Nagadhara1, S Ramesh, I C Pasalu
1Centre for Plant Molecular Biology, Osmania University, Hyderabad-500 007, India.
Plant Biotechnology Journal
|December 13, 2006
Summary
This study optimized genetic transformation in indica rice to resist sap-sucking insects. Transgenic rice expressing the snowdrop lectin gene (gna) showed significant resistance to brown planthopper (BPH) and green leafhopper (GLH).
Area of Science:
- Agricultural Science
- Molecular Biology
- Entomology
Background:
- Indica rice is susceptible to sap-sucking insects like brown planthopper (BPH) and green leafhopper (GLH).
- Genetic engineering offers a potential strategy to enhance insect resistance in crops.
Purpose of the Study:
- To develop transgenic indica rice with enhanced resistance to BPH and GLH using Agrobacterium-mediated transformation.
- To confirm the stable integration and expression of introduced genes in rice.
Main Methods:
- Agrobacterium-mediated transformation of indica rice using embryogenic calli.
- Co-cultivation with Agrobacterium strain LBA4404 harboring pSB111-bar-gna plasmid.
- Selection of transgenic events using phosphinothricin and confirmation via PCR, Southern, Northern, and Western blot analyses.
Main Results:
- Stable integration of snowdrop lectin gene (gna) and herbicide resistance gene (bar) confirmed in transgenic rice (T0).
- Expression of the gna gene was detected in transgenic plants.
- Co-segregation of gna and bar transgenes observed in T1 and T2 generations (3:1 ratio).
- Transgenic rice lines exhibited substantial resistance to both BPH and GLH.
Conclusions:
- Agrobacterium-mediated transformation is effective for developing insect-resistant indica rice.
- The snowdrop lectin gene confers significant resistance to major sap-sucking pests in rice.
- This represents the first report of transgenic indica rice with high resistance to both BPH and GLH.
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