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Updated: Jul 18, 2026

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Published on: January 19, 2021
A role for Phospholipase D in Drosophila embryonic cellularization
Mary LaLonde1, Hilde Janssens, Suyong Yun
1Department of Pharmacology and Center for Developmental Genetics, Stony Brook University, Stony Brook, NY 11794-5140, USA. mary.lalonde@uhmc.sunysb.edu <mary.lalonde@uhmc.sunysb.edu>
Phospholipase D (Pld) is crucial for Drosophila embryo cellularization, a key step in development. Its absence disrupts Golgi structure and vesicle transport, leading to developmental arrest.
Area of Science:
- Developmental Biology
- Cell Biology
- Molecular Signaling
Background:
- Drosophila embryo cellularization involves synchronous cytokinesis.
- Membrane extension occurs via fusion of Golgi-derived vesicles.
Purpose of the Study:
- Investigate the role of Phospholipase D (Pld) in Drosophila embryo cellularization.
- Elucidate the mechanism by which Pld regulates this process.
Main Methods:
- Gene targeting to create Pld-deficient Drosophila.
- Analysis of embryonic development and cellularization.
- Microscopy to examine Golgi structure and vesicle trafficking.
Main Results:
- Loss of Pld function significantly hinders cellularization.
- Pld deficiency leads to early embryonic developmental arrest.
- Absence of Pld results in abnormal Golgi structure and impaired vesicle trafficking.
Conclusions:
- Phospholipase D (Pld) plays a vital role in promoting vesicle trafficking during cellularization.
- Pld is essential for the fusion-competence of Golgi-derived vesicles.
- Pld signaling is critical for successful cytokinesis in the early Drosophila embryo.
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