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Prototheca zopfii genotype 2: the causative agent of bovine protothecal mastitis?

Asja Möller1, Uwe Truyen, Uwe Roesler

  • 1Institute of Animal Hygiene and Veterinary Public Health, Faculty of Veterinary Medicine, University of Leipzig, An den Tierklinken 1, D-04103 Leipzig, Germany.

Veterinary Microbiology
|December 13, 2006
PubMed

Insights

This study investigated bovine mastitis isolates, identifying Prototheca zopfii genotype 2 as the primary cause of bovine Prototheca mastitis through genetic analysis.

Area of Science:

  • Veterinary Microbiology
  • Molecular Epidemiology
  • Bovine Diseases

Background:

  • Protothecal mastitis in cattle is an emerging concern.
  • The causative agent, Prototheca zopfii, has been reclassified.
  • Genetic diversity within Prototheca species is not fully understood.

Purpose of the Study:

  • To genetically characterize Prototheca isolates from bovine mastitis cases.
  • To determine the specific genotype responsible for bovine Prototheca mastitis.
  • To clarify the epidemiology of this disease.

Main Methods:

  • Genetic analysis of 30 Prototheca zopfii mastitis isolates.
  • Utilized 18S ribosomal DNA (rDNA) sequencing for differentiation.
  • Developed and applied genotype-specific Polymerase Chain Reaction (PCR) and Restriction Fragment Length Polymorphism (RFLP) assays.

Main Results:

  • The 18S rDNA analysis differentiated Prototheca zopfii into two genotypes and identified Prototheca blaschkeae.
  • All 30 mastitis isolates were successfully assigned to Prototheca zopfii genotype 2.
  • This suggests a specific etiological agent for bovine Prototheca mastitis.

Conclusions:

  • Prototheca zopfii genotype 2 is the predominant etiological agent in bovine mastitis.
  • Genotype-specific diagnostic tools are effective for epidemiological studies.
  • Further research into the transmission and control of this genotype is warranted.

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