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Establishing a Porcine Ex Vivo Cornea Model for Studying Drug Treatments against Bacterial Keratitis
Published on: May 12, 2020
Analysis of bacterial cultures in infectious keratitis, 1997 to 2004
David L Yeh1, Sandra S Stinnett, Natalie A Afshari
1Department of Ophthalmology, Duke University Eye Center, Duke University Medical Center, Durham, North Carolina 27710, USA.
Purpose:
To review bacterial culture results in infectious keratitis at a tertiary referral center.
Design:
Retrospective case series.
Methods:
Analysis of Gram stain, bacterial culture, and targeted antibiotic sensitivities of all cases of presumed infectious keratitis at Duke University Eye Center from 1997 to 2004 (n = 453).
Results:
Cultures were positive in 307 cases (68%); 21% demonstrated polymicrobial growth. Among 388 isolates, 81% were Gram-positive. Gram stain results were available in 334 cases, demonstrating 18% sensitivity and 94% specificity. Antibiotic sensitivities revealed 24% resistance of tested S. aureus isolates to cefazolin, and <5% resistance of tested gram-negative isolates to various antibiotics, with no statistically significant increase from 1997 to 2004.
Conclusions:
In this study, the microbial profile has remained stable from 1997 to 2004. Gram stain studies were of poor sensitivity, while culture yields were significantly higher and demonstrated a relatively high polymicrobial infection rate. Increasing antibiotic resistance was not clearly demonstrated.
