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Proteolipid DM-20 predominates over PLP in peripheral nervous system
D Pham-Dinh1, M C Birling, G Roussel
1Laboratoire des Protéines (URA 1188 CNRS), Université de Paris V, France.
Neuroreport
|February 1, 1991
Summary
The proteolipid (PLP) and its isoform DM-20 are found in peripheral nerves. DM-20 is more prevalent than PLP in rat sciatic nerves, unlike in the central nervous system.
Area of Science:
- Neuroscience
- Cell Biology
- Biochemistry
Background:
- The major myelin proteolipid (PLP) is primarily known for its role in the central nervous system (CNS).
- Expression of CNS proteins in the peripheral nervous system (PNS) suggests conserved or novel functions.
Purpose of the Study:
- To investigate the presence and relative abundance of PLP and its isoform DM-20 in the PNS, specifically rat sciatic nerves.
- To compare the expression patterns of PLP and DM-20 in the PNS with those in the CNS.
Main Methods:
- Immunoblot analysis using site-specific antibodies against PLP and DM-20 on peripheral nerve extracts.
- Polymerase Chain Reaction (PCR) amplification of cDNAs synthesized from sciatic nerve RNA to characterize proteolipid expression.
Main Results:
- Evidence confirms the presence of DM-20, an isoform of PLP, in rat sciatic nerves.
- DM-20 predominates over PLP in peripheral nerve myelin, a pattern inverse to that observed in CNS myelin.
- PCR analysis corroborated the immunoblot findings regarding proteolipid expression.
Conclusions:
- The proteolipid DM-20 is expressed in the peripheral nervous system (PNS).
- The expression profile of PLP isoforms differs between the CNS and PNS.
- The functional significance of these proteolipids in the PNS requires further investigation.