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Related Experiment Videos

Systematic variation in mRNA 3'-processing signals during mouse spermatogenesis.

Donglin Liu1, J Michael Brockman, Brinda Dass

  • 1The Jackson Laboratory, 600 Main Street, Bar Harbor, ME 04609, USA.

Nucleic Acids Research
|December 13, 2006
PubMed
Summary

Male germ cell maturation involves specialized gene processing. Alternative 3' processing and shorter 3' untranslated regions (3'-UTRs) in messenger RNAs (mRNAs) are key features during spermatogenesis.

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Area of Science:

  • Molecular Biology
  • Genetics
  • Reproductive Biology

Background:

  • Gene expression and processing are highly specialized during mouse male germ cell maturation (spermatogenesis).
  • Previous studies suggested a high incidence of alternative 3 eal-processing in male germ cell messenger RNAs (mRNAs), including reduced usage of the canonical polyadenylation signal (AAUAAA).

Purpose of the Study:

  • To identify 3 eal-processing sites used at various stages of spermatogenesis and in Sertoli cells.
  • To assess differences in 3 eal-processing characteristics and regulatory elements during male germ cell development.

Main Methods:

  • Utilized EST libraries from mouse testicular cells (spermatogonia, spermatocytes, round spermatids, Sertoli cells).
  • Employed a novel method for comparing degenerate regulatory elements between sequence samples.

Related Experiment Videos

  • Analyzed 3 eal-untranslated regions (3 eal-UTRs) for truncation and differences in processing sites.

    • Main Results:

    • Identified significant changes in the use of putative 3 eal-processing regulatory sequence elements across all spermatogenic cell types.
    • Observed a trend towards truncated 3 eal-UTRs, most pronounced in round spermatids.
    • Sertoli cells showed minimal 3 eal-UTR truncation and no significant difference in regulatory sequences.

    Conclusions:

    • Spermatogenesis exhibits developmental differences in polyadenylation site choice and regulatory elements.
    • Alternative 3 eal-processing and 3 eal-UTR truncation are specific features of male germ cell development.
    • Identified specific genes undergoing alternative 3 eal-processing during meiosis and postmeiotic development.