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A common epitope is shared by ciliary rootlets and cell-cell adherens junctions in ciliated ependymal cells
S Peraldi-Roux1, C Klotz, B Nguyen-Thanh-Dao
1Laboratoire de Neurobiologie Endocrinologique, URA 1197 CNRS, Université de Montpellier II-Sciences, France.
Journal of Cell Science
|June 1, 1991
Summary
A novel monoclonal antibody, CC.310, identifies a ciliary rootlet protein in ependymal cells. This antibody aids in studying ciliogenesis and reveals the protein
Area of Science:
- Cell Biology
- Neuroscience
Background:
- Ciliary rootlets are crucial structures in ciliated cells.
- Understanding the molecular composition of ciliary rootlets is essential for cell biology.
Purpose of the Study:
- To investigate the presence and localization of a ciliary rootlet protein using monoclonal antibody CC.310.
- To characterize the antigen recognized by mAb CC.310 in hypothalamic ependymal cultures.
Main Methods:
- Immunoblotting
- Light and electron immunocytochemistry
- Immunofluorescence studies
Main Results:
- Monoclonal antibody CC.310 recognized a unique antigen in ependymal cultures, localized to ciliary rootlets and intercellular junctions.
- The antigen was associated with the Triton X-100-insoluble fraction and migrated to 94K.
- mAb CC.310 facilitated observation of ciliogenesis and confirmed the protein's presence in ciliary rootlets and near adherens-type junctional complexes.
Conclusions:
- Monoclonal antibody CC.310 is a suitable marker for ciliated ependymocytes.
- Proteins sharing a common epitope with the ciliary rootlet protein are located in both rootlets and junctional complexes.
- The study provides insights into the molecular composition and localization of ciliary rootlet proteins.
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