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Updated: Jul 18, 2026

A Rapid and Quantitative Fluorimetric Method for Protein-Targeting Small Molecule Drug Screening
Published on: October 16, 2015
Interaction between fluoroquinolones and bovine serum albumin studied by affinity capillary electrophoresis
Shiwang Liu1, Li-Wei Zhang, Xin-Xiang Zhang
1Department of Biology, Huanggang Normal University, Hubei, China.
Abstract:
The interactions between eight fluoroquinolone antibiotics (ciprofloxacin, enoxacin, fleroxacin, levofloxacin, lomefloxacin, norfloxacin, ofloxacin, pefloxacin) and bovine serum albumin (BSA) were studied by affinity capillary electrophoresis (ACE). The binding constants were estimated by the change of migration times of the analytes through the change of concentration of BSA in the buffer solution. The yield binding constants were between 3.19 x 10(4) and 1.21 x 10(5) M(-1). These were related with the structures of fluoroquinolones, and agreed with the results obtained by other techniques. The obtained binding constants may help us in gaining some insights on possible drug/protein interactions and in early evaluation of the drugs' pharmacokinetic profiles during drug discovery.
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