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Updated: Jul 18, 2026

Protein Purification-free Method of Binding Affinity Determination by Microscale Thermophoresis
Published on: August 15, 2013
A hollow fiber solvent microextraction approach to measure drug-protein binding
Huafeng Fu1, Jiyu Guan, James J Bao
1School of Pharmaceutical Science and Technology, Tianjin University, China.
Abstract:
A new direct method has been developed to determine protein-drug binding based on hollow fiber membrane solvent microextraction. Hollow-fiber membrane solvent microextraction coupled with high-performance chromatography with UV detection was employed to evaluate the binding characteristics of drugs to bovine serum albumin (BSA) and blood serum. It was found that the BSA and matrix in the blood serum did not interfere with the measurement. The method is simple and fast. It lacks the drawbacks of some conventional analytical techniques, such as taking much long time and requiring large volume sample consumption.
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