Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Searching for sequence directed mutagenesis in eukaryotes.

Emmanuel D Ladoukakis1, Adam Eyre-Walker

  • 1Centre for Study of Evolution and School of Life Sciences, University of Sussex, Brighton BN1 9QG, UK.

Journal of Molecular Evolution
|December 13, 2006
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Nucleotide diversity is a poor predictor of short-term adaptive potential.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Population Genetic Study of <i>vitellogenin</i> in Honey Bees (<i>Apis mellifera</i>) With European Ancestry Identifies Two Ancestral Genetic Backgrounds.

Ecology and evolution·2026
Same author

Levels of additive genetic variation vary substantially between species.

PLoS biology·2026
Same author

Evaluating the phylogenetic signal of morphosyntax.

Poznan studies in contemporary linguistics : PSiCL·2026
Same author

A Comparative Analysis of Long-Term Effective Population Sizes Across Eukaryotes.

Molecular ecology·2026
Same author

Pervasive non-random mitochondrial DNA heteroplasmy in the hybrid water frog Pelophylax esculentus.

BMC ecology and evolution·2025
Same journal

Deep Dive into Evolution: How Cetaceans Adapt Their Anticoagulant Genes for Underwater Survival.

Journal of molecular evolution·2026
Same journal

Sensing Underwater: Diversifying Selection, Convergent Evolution and Inactivation in Sensory Receptors' Genes of Aquatic Mammals.

Journal of molecular evolution·2026
Same journal

Synonymous Codons as Potential Contributors to Chromatin Stability and Gene Body Methylation in Plants.

Journal of molecular evolution·2026
Same journal

Convergent Functional Genomic Evolution Underlying Repeated Freshwater Colonization in Cetaceans.

Journal of molecular evolution·2026
Same journal

Conditions Enabling the Persistence of Cooperating Synthetase, Ligase, and Mutation-Inhibitor Catalytic Polymers.

Journal of molecular evolution·2026
Same journal

Lineage-Specific Diversification of Nucleoporin Nup98 Genes in Ciliates and Its Evolutionary Implications for the Nuclear Dualism.

Journal of molecular evolution·2026
See all related articles

Sequence directed mutagenesis, where DNA repeats correct each other, is common in prokaryotes but rare in eukaryotes. Our study found little evidence for this mutation process in eukaryotic protein-coding genes.

Area of Science:

  • Genetics and Molecular Biology
  • Evolutionary Biology
  • Genomic Instability

Background:

  • Sequence directed mutagenesis (SDM) is a known mechanism in prokaryotes.
  • SDM involves imperfect DNA repeats self-correcting into perfect repeats, causing mutations.
  • This process has been linked to human genetic disorders.

Purpose of the Study:

  • To investigate the prevalence of sequence directed mutagenesis in eukaryotic protein-coding sequences.
  • To determine if SDM is a significant factor in eukaryotic genome evolution and disease.

Main Methods:

  • Analysis of extensive DNA sequence data from multiple eukaryotic species (humans, mice, Drosophila, nematodes, yeast, Arabidopsis).
  • Application of two distinct statistical tests to detect evidence of SDM.

Related Experiment Videos

  • Focus on protein-coding regions to assess functional impact.

    • Main Results:

    • Little evidence supporting the occurrence of sequence directed mutagenesis was found across the studied eukaryotic genomes.
    • The prevalence of SDM appears to be significantly lower in eukaryotes compared to prokaryotes.
    • No strong correlation was identified between SDM and observed mutations in eukaryotic protein-coding sequences.

    Conclusions:

    • Sequence directed mutagenesis is not a prevalent mechanism in the protein-coding sequences of eukaryotes.
    • The role of SDM in eukaryotic genetic diseases may be overestimated or coincidental.
    • Further research may be needed to explore alternative mutation mechanisms in eukaryotes.