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Micropatterned surfaces for controlling cell adhesion and rolling under flow.

Divya D Nalayanda1, Mahendran Kalukanimuttam, David W Schmidtke

  • 1University of Oklahoma Bioengineering Center, School of Chemical, Biological, and Materials Engineering, University of Oklahoma, Norman, OK 73019, USA.

Biomedical Microdevices
|December 13, 2006
PubMed
Summary

Microfluidic patterning precisely controls cell adhesion and rolling on vascular walls. This cost-effective technique fabricates patterned surfaces for studying inflammation and thrombosis under physiological flow conditions.

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Area of Science:

  • Biomedical Engineering
  • Cell Biology
  • Biophysics

Background:

  • Cell adhesion and rolling on vascular walls are crucial processes in inflammation and thrombosis.
  • Understanding these interactions is vital for developing therapeutic strategies.

Purpose of the Study:

  • To demonstrate the feasibility of microfluidic patterning for controlling cell adhesion and rolling.
  • To create patterned surfaces for studying cell-wall interactions under physiological flow.

Main Methods:

  • Fabricated surfaces with well-defined patterns of adhesion molecules using microfluidic channels with controlled line widths and spacing.
  • Patterned surfaces with P-selectin, E-selectin, and von Willebrand Factor.
  • Controlled surface ligand density by varying incubation solution concentration.

Main Results:

  • Successfully controlled adhesion and rolling of neutrophils, Chinese Hamster Ovary cells, and platelets.
  • Demonstrated that varying surface ligand density affects cell rolling velocity.
  • Achieved simultaneous control of leukocyte and platelet rolling on surfaces patterned with both P-selectin and von Willebrand Factor.

Conclusions:

  • Microfluidic patterning offers an effective and inexpensive method for fabricating patterned surfaces for cell rolling assays.
  • This technique allows precise control over cell adhesion and rolling dynamics under physiological flow conditions.
  • Enables simultaneous study of multiple cell types and adhesion molecules, advancing research in inflammation and thrombosis.