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Related Experiment Videos

Metal-enhanced fluorescence from gold surfaces: angular dependent emission.

Kadir Aslan1, Stuart N Malyn, Chris D Geddes

  • 1Institute of Fluorescence, Laboratory for Advanced Medical Plasmonics, Medical Biotechnology Center, University of Maryland Biotechnology Institute, 725 West Lombard St, Baltimore, MD 21201, USA.

Journal of Fluorescence
|December 13, 2006
PubMed
Summary
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Large gold colloids demonstrate Metal-Enhanced Fluorescence (MEF), boosting FITC-HSA emission up to 2.5-fold. Larger 200 nm colloids showed greater enhancement than 40 nm ones, suggesting potential for stable MEF applications.

Area of Science:

  • Plasmonics
  • Nanotechnology
  • Biophotonics

Background:

  • Metal-Enhanced Fluorescence (MEF) is a phenomenon where fluorophores near noble metal nanostructures exhibit increased emission intensity.
  • Gold nanoparticles are increasingly explored for their plasmonic properties and potential in biosensing and imaging.
  • Previous MEF studies often utilized silver nanostructures, which can be less stable over time.

Purpose of the Study:

  • To report the first observation of MEF using large gold colloids (40 and 200 nm).
  • To investigate the angular dependence of MEF from gold colloids.
  • To compare the MEF efficiency of different sized gold colloids and assess their suitability for applications.

Main Methods:

  • Homogeneous deposition of 40 nm and 200 nm gold colloids onto glass substrates.

Related Experiment Videos

  • Adsorption of FITC-HSA (fluorescein isothiocyanate-labeled human serum albumin) onto the gold colloid surfaces.
  • Measurement of angular-dependent fluorescence emission using a rotating stage to evaluate MEF at all spatial angles.

    • Main Results:

    • A significant increase in fluorescence intensity (up to 2.5-fold) of FITC-HSA was observed on gold colloids compared to bare glass substrates, particularly at a 270-degree angle.
    • The Radiating Plasmon Model explains the observed MEF through energy transfer between excited fluorophores and surface plasmons of gold colloids.
    • Larger, 200 nm gold colloids exhibited higher fluorescence enhancement than 40 nm colloids, attributed to the scattering component of their extinction spectrum.

    Conclusions:

    • Large gold colloids are effective substrates for achieving Metal-Enhanced Fluorescence.
    • The observed MEF is consistent with the Radiating Plasmon Model, involving coupled fluorophore-plasmon interactions.
    • Gold colloids offer a promising, stable alternative to silver for MEF applications, particularly those requiring long-term use and storage.