Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Videos

A mouse model for food allergy using intraperitoneal sensitization.

Rebecca J Dearman¹, Ian Kimber

¹Syngenta Central Toxicology Laboratory, Alderley Park, Macclesfield, Cheshire SK10 4TJ, UK. rebecca.dearman@syngenta.com

Methods (San Diego, Calif.)

|December 13, 2006

Summary

This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Provocation of occupational asthma by non-specific irritation and its relevance for the classification of chemicals as respiratory allergens: a case study of methyl methacrylate.

Regulatory toxicology and pharmacology : RTP·2026

Same author

Derivation of a Point of Departure using NAMs for application in Quantitative Risk Assessment of fragrance materials.

Regulatory toxicology and pharmacology : RTP·2026

Same author

Occupational asthma: dust exposure as a contributory factor and implications for classification of respiratory sensitisers.

Regulatory toxicology and pharmacology : RTP·2025

Same author

An extended Reference Chemical Potency List (RCPL) for characterising the performance of New Approach Methodologies (NAMs) in measuring the skin sensitisation potency of fragrance chemicals.

Regulatory toxicology and pharmacology : RTP·2025

Same author

Accurate regulatory classification of chemical respiratory allergens: The case for robust characterisation of causation.

Regulatory toxicology and pharmacology : RTP·2025

Same author

Comment regarding: 'On the solubility of azodicarbonamide in water/DMSO mixtures: an experimental and computational study' (2024), by Macetti <i>et al.</i>

Royal Society open science·2024

Assessing food allergy potential requires new methods. This study explores using IgE antibody induction in BALB/c mice as a promising model for evaluating food protein allergenicity.

Area of Science:

Food science
Immunology
Toxicology

Background:

Food allergy is a significant public health concern.
Transgenic crops introduce novel food proteins, necessitating robust allergenicity assessment.
Distinguishing immunogenicity from allergenicity (IgG vs. IgE response) is crucial.

Purpose of the Study:

To evaluate a novel animal model for characterizing food protein allergenicity.
To assess the induction of specific IgE antibodies as a measure of allergenic potential.
To compare this method with existing experimental models.

Main Methods:

Immunization of BALB/c mice via intraperitoneal injection.
Measurement of specific IgE antibody induction.
Utilizing homologous passive cutaneous anaphylaxis for assessment.

Related Experiment Videos

Comparison with other experimental models, considering exposure routes, adjuvants, and endpoints.

Main Results:

The BALB/c mouse model demonstrated potential in assessing food protein allergenicity.
Specific IgE antibody induction correlated with allergenic potential.
The method's progress and comparisons with other models were summarized.

Conclusions:

The described mouse model shows promise for evaluating the allergenic potential of food proteins.
Further research and validation are needed for a definitive animal model.
This approach contributes to the safety assessment of novel food sources.