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Rapid Mix Preparation of Bioinspired Nanoscale Hydroxyapatite for Biomedical Applications
Published on: February 23, 2017
Mechanistic model for adsorption of immunoglobulin on hydroxyapatite
Paul K Ng1, Jie He, Pete Gagnon
1Process Applications, Process Chromatography Division, Life Sciences Group, Bio-Rad Laboratories, Hercules, CA 94547, USA. paul_ng@bio-rad.com <paul_ng@bio-rad.com>
Abstract:
A model is developed for correlating the adsorption of protein to hydroxyapatite in terms of pH, phosphate and imidazole. It provides a basis for manipulating the chromatography conditions so as to obtain the best possible fractionation. The adsorption mechanism is apparently a function of two calcium affinity interactions and cation exchange. We used immunoglobulins and a fusion protein with or without a poly(6)histidine tag for our investigation. The results are presented and discussed in the present study.
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