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Purification of hematopoietic stem cells using the side population

K K Lin1, Margaret A Goodell

  • 1Stem Cells and Regenerative Medicine Center, Baylor College of Medicine, Houston, Texas, USA.

Methods in Enzymology
|December 13, 2006
PubMed

Hematopoietic stem cells (HSCs) primarily reside in bone marrow, are defined by their ability to maintain blood homeostasis, and replenish themselves through self-renewal. Although HSC purification schemes vary from laboratory to laboratory, the resulting cell populations are similar, if not the same. This chapter will discuss different enrichment methods for HSCs and provide a detailed protocol for staining HSC with Hoechst 33342 for the side population (SP).

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