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Detection of human immunodeficiency virus type 1 genomic RNA in plasma samples by reverse-transcription polymerase
P Bagnarelli1, S Menzo, A Manzin
1Institute of Microbiology, University of Ancona Medical School, Italy.
Journal of Medical Virology
|June 1, 1991
Abstract:
An application of the polymerase chain reaction (PCR) to the direct detection of human immunodeficiency virus type 1 (HIV-1) viremia is described. The amplification of specific HIV-1 sequences of gag and env viral genes was carried out after the reverse-transcription of plasma samples (plasma RT-PCR) from seropositive subjects. The assay is faster and cheaper than detection of specific HIV-1 transcripts from peripheral blood mononuclear cells by RT-PCR. The data suggest that HIV-1 viremia is detectable by plasma RT-PCR in a large proportion of seropositive individuals.