Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Intracellular metal detectors.

Amy M Barrios1

  • 1Department of Chemistry, University of Southern California, Los Angeles, California 90089, USA. amy.barrios@usc.edu

ACS Chemical Biology
|December 14, 2006
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Inhibition of PHPT1 by phenylarsonic acids.

Dalton transactions (Cambridge, England : 2003)·2026
Same author

Multipartite Fluorogenic Sensors for Monitoring Tyrosine Phosphatase Activity.

Chembiochem : a European journal of chemical biology·2024
Same author

The mechanism of covalent inhibition of LAR phosphatase by illudalic acid.

Bioorganic & medicinal chemistry letters·2024
Same author

Derivatives of the Fungal Natural Product Illudalic Acid Inhibit the Activity of Protein Histidine Phosphatase PHPT1.

ChemMedChem·2023
Same author

Inhibitor Screen Identifies Covalent Inhibitors of the Protein Histidine Phosphatase PHPT1.

ACS medicinal chemistry letters·2022
Same author

Small Molecules Targeting PTPσ-Trk Interactions Promote Sympathetic Nerve Regeneration.

ACS chemical neuroscience·2022

Accurately measuring intracellular metal ions, especially zinc, is vital but challenging. A new cell-permeable, ratiometric fluorescence resonance energy transfer (FRET) biosensor now enables quantification of the exchangeable zinc pool in cells.

Area of Science:

  • Biochemistry
  • Cell Biology
  • Analytical Chemistry

Background:

  • Metal ions are essential for numerous biological processes.
  • Accurate intracellular concentration measurement of metal ions, both bound and exchangeable, is of significant interest.
  • Quantifying readily exchangeable transition metal ions, such as zinc, presents challenges due to low concentrations, interference, and probe delivery.

Discussion:

  • This study introduces a novel cell-permeable, ratiometric, fluorescence resonance energy transfer (FRET) based zinc biosensor.
  • The developed biosensor overcomes previous limitations in measuring intracellular metal ion concentrations.
  • This advancement facilitates the first-time quantification of the intracellular exchangeable zinc pool.

Key Insights:

  • The new FRET biosensor allows for precise measurement of intracellular exchangeable zinc.

Related Experiment Videos

  • The ratiometric nature of the sensor enhances accuracy and reduces interference.
  • This technology provides a crucial tool for studying zinc's biological roles at the cellular level.

    • Outlook:

    • Future research can utilize this biosensor to explore the dynamic changes and functions of intracellular zinc in various biological contexts.
    • This method holds potential for broader applications in measuring other essential metal ions.
    • Further development could lead to in vivo applications for real-time monitoring of metal ion homeostasis.