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Microsatellite DNA Genotyping and Flow Cytometry Ploidy Analyses of Formalin-fixed Paraffin-embedded Hydatidiform Molar Tissues
Published on: October 20, 2019
Differential diagnosis between complete and partial mole using a TSSC3 antibody: correlation with DNA polymorphic
1Department of Gynecology, National Hokkaido Cancer Center, Sapporo City, Japan. hidekato@sap-cc.go.jp
The Journal of Reproductive Medicine
|December 15, 2006
Summary
Complete silencing of TSSC3 gene expression effectively distinguishes complete hydatidiform mole (CHM) from partial hydatidiform mole (PHM). This finding provides a novel diagnostic strategy for molar lesions.
Area of Science:
- Reproductive biology
- Molecular genetics
- Gynecologic pathology
Background:
- Molar pregnancies, including complete hydatidiform mole (CHM) and partial hydatidiform mole (PHM), require accurate differential diagnosis.
- Imprinted genes play crucial roles in placental development and are often dysregulated in molar pregnancies.
Purpose of the Study:
- To evaluate the diagnostic utility of maternally expressed, imprinted genes for differentiating CHM from PHM.
- To identify specific imprinted genes with altered expression patterns in CHM.
Main Methods:
- Microarray analysis was used to assess imprinted gene expression in CHM.
- Western blot analysis and immunohistochemistry were performed on 20 CHMs and 10 PHMs to validate expression patterns of key genes.
Main Results:
- TSSC3, SLC22A1L, KCNQ1, and decorin showed down-regulation in CHM.
- Complete loss of TSSC3 expression was observed in all confirmed CHM cases.
- Normal TSSC3 expression was detected in all confirmed PHM cases, consistent across Western blot and immunohistochemistry.
Conclusions:
- Complete silencing of TSSC3 expression serves as a reliable biomarker for CHM.
- This diagnostic approach offers a convenient and novel method for diagnosing molar lesions.
