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[Studies on extraction, isolation and composition of Lycium barbarum polysaccharides]
1School of Life Science and Technology, China Pharmaceutical University, Nanjing 210009, China.
Objective:
To study the extraction, isolation and composition of Lycium barbarum polysaccharides (LBP).
Method:
LBP was extracted from L. barbarum with water, isolationed and purified by DEAE ion-exchange cellulose and gel chromatography, and their structural composition was studied by means of SDS-PAGE gel electrophoresis, GC, amino acid automatic analysis, etc.
Result:
Pure LBP has four water solubie polysaccharides, M W was 1.524 x 10(5). LBP was composed of 6 kinds of monosaccharides (Ara, Rha, Xyl, Man, Gal and Glc), galacturonic acid and 18 kinds of amino acids.
Conclusion:
LBP is a kind of complex polysaccharides consisting of acidic heteropolysaccharides and polypeptide or protein, and LBP has Glycan-O-Ser glycopeptide structures.
