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Related Experiment Videos

Acidic fibroblast growth factor in the developing rat embryo.

Y M Fu1, P Spirito, Z X Yu

  • 1Cardiology Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892.

The Journal of Cell Biology
|September 1, 1991
PubMed
Summary

Acidic fibroblast growth factor (aFGF) and basic fibroblast growth factor (bFGF) are widely distributed in developing rat embryos, contrary to previous assumptions. Both factors show similar patterns in neuroectodermal and mesodermal tissues during differentiation.

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Area of Science:

  • Developmental Biology
  • Molecular Biology
  • Cell Biology

Background:

  • Basic fibroblast growth factor (bFGF) is a known mitogen and mesoderm inducer.
  • Acidic fibroblast growth factor (aFGF) was previously thought to have a restricted neural distribution and be absent in early embryos.

Purpose of the Study:

  • To investigate the cellular and tissue distributions of aFGF and bFGF in developing rat embryos.
  • To compare the expression patterns of aFGF and bFGF during embryonic development.

Main Methods:

  • Immunoblotting and immunochemistry were employed to detect aFGF and bFGF.
  • Analysis was performed on rat embryos at developmental stages 11-20 days.
  • Urea pretreatment was used to resolve high molecular mass forms of FGFs.

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Main Results:

  • High molecular mass forms of immunoreactive aFGF and bFGF were detected in rat embryo extracts.
  • Both aFGF and bFGF showed widespread distribution in neuroectodermal and mesodermal cells (cytoplasm, nuclei, extracellular matrix).
  • Endoderm-derived cells were negative for both aFGF and bFGF immunoreactivity.
  • Expression patterns of aFGF and bFGF were similar across different tissues and developmental stages, increasing with neural cell differentiation.

Conclusions:

  • Contrary to prior reports, aFGF and bFGF are present in the developing rat embryo.
  • Both factors exhibit similar and widespread distribution in neuroectodermal and mesodermal tissues.
  • Their distribution changes correlate with cellular and tissue differentiation processes.