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Two-parameter mobile phase optimization for the simultaneous high-performance liquid chromatographic determination of
1Department of Physiology, Faculty of Biology, University of Santiago, Galicia, Spain.
Journal of Chromatography
|June 28, 1991
Abstract:
A new high-pressure liquid chromatography method with electrochemical detection is described that allows the simultaneous determination of dopamine, serotonin, 3,4-dihydroxyphenylacetic acid, 3-methoxy-4-hydroxyphenylacetic acid, 5-hydroxytryptophan and 5-hydroxyindoleacetic acid in microdissected nuclei from individual rat brains. No sample pre-treatment steps are required. Resolution and analysis time were optimized by a simple limited optimization procedure, involving two-parameter factorial design.