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Related Experiment Video

Updated: Jul 18, 2026

Isolation of Peritoneum-derived Mast Cells and Their Functional Characterization with Ca2+-imaging and Degranulation Assays

Isolation of Peritoneum-derived Mast Cells and Their Functional Characterization with Ca²⁺-imaging and Degranulation Assays

Published on: July 4, 2018

Progesterone inhibits mast cell secretion.

M Vasiadi¹, D Kempuraj, W Boucher

¹Department of Pharmacology and Experimental Therapeutics, Tufts University School of Medicine, Tufts-New England Medical Center, Boston, MA, USA.

International Journal of Immunopathology and Pharmacology

|December 15, 2006

Summary

Progesterone inhibits histamine secretion from mast cells, potentially explaining why inflammatory conditions improve during pregnancy. This finding offers new insights into mast cell regulation and reproductive health.

More Related Videos

Investigating Mast Cell Secretory Granules; from Biosynthesis to Exocytosis

Investigating Mast Cell Secretory Granules; from Biosynthesis to Exocytosis

Published on: January 26, 2015

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Last Updated: Jul 18, 2026

Isolation of Peritoneum-derived Mast Cells and Their Functional Characterization with Ca2+-imaging and Degranulation Assays

Isolation of Peritoneum-derived Mast Cells and Their Functional Characterization with Ca²⁺-imaging and Degranulation Assays

Published on: July 4, 2018

Investigating Mast Cell Secretory Granules; from Biosynthesis to Exocytosis

Investigating Mast Cell Secretory Granules; from Biosynthesis to Exocytosis

Published on: January 26, 2015

Area of Science:

Immunology
Endocrinology
Cell Biology

Background:

Mast cells mediate allergic reactions by releasing inflammatory mediators.
Mast cells are implicated in conditions like IBS and migraines, which fluctuate with hormonal changes.
Estrogen enhances mast cell secretion, while its role in pregnancy-related symptom reduction is unclear.

Purpose of the Study:

To investigate the effect of progesterone on mast cell secretion.
To explore the potential role of progesterone in regulating inflammatory conditions during pregnancy.

Main Methods:

Purified rat peritoneal mast cells were used.
Cells were stimulated immunologically or with Substance P (SP).
Histamine secretion was measured, and electron microscopy was employed.

Main Results:

Progesterone (100 nM) significantly inhibited histamine secretion.
Cholesterol did not affect histamine secretion.
Electron microscopy confirmed the inhibitory effect of progesterone on mast cell degranulation.

Conclusions:

Progesterone regulates mast cell secretion, specifically inhibiting histamine release.
This hormonal regulation may explain the reduced symptoms of certain inflammatory diseases during pregnancy.
Further research into progesterone's role in mast cell-mediated conditions is warranted.