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Involvement of the ERK1/2 MAPK pathway in insulin-induced S6K1 activation in avian cells

Sophie Duchêne1, Estelle Audouin, Sabine Crochet

  • 1UR83 Recherches Avicoles, INRA, 37380 Nouzilly, France.

Domestic Animal Endocrinology
|December 15, 2006
PubMed

Insights

Insulin activates the ERK1/2 MAPK pathway to control p70 S6 kinase (S6K1) in avian muscle cells. This pathway is crucial for protein synthesis regulation in chickens, especially in myoblasts.

Area of Science:

  • * Molecular Biology
  • * Endocrinology
  • * Cell Signaling

Background:

  • * Insulin regulates protein synthesis via the PI3K pathway in mammals.
  • * Avian muscle shows insulin-activated S6K1 despite PI3K pathway insensitivity.
  • * Mitogen-activated protein kinase (MAPK) is an insulin-sensitive pathway.

Purpose of the Study:

  • * To investigate the role of the ERK1/2 MAPK pathway in avian S6K1 regulation by insulin.
  • * To characterize ERK1/2 MAPK in chicken tissues and cells.

Main Methods:

  • * Characterization of ERK1/2 MAPK isoforms in chicken tissues.
  • * In vivo activation studies of ERK2 by insulin in chicken muscle.
  • * Investigation of insulin signaling in chicken hepatoma (LMH) cells and primary myoblasts.
  • * Use of U0126, an ERK MAPK pathway inhibitor.

Main Results:

  • * ERK2 was the sole detected ERK isoform in avian tissues.
  • * Insulin activated ERK2 in vivo in chicken muscle.
  • * Insulin stimulated ERK2 and S6K1 phosphorylation and kinase activity in LMH cells and myoblasts.
  • * U0126 abolished insulin-induced S6K1 changes in myoblasts but only partially in LMH cells.

Conclusions:

  • * The ERK1/2 MAPK pathway is involved in insulin control of S6K1 in chicken cells.
  • * This involvement is particularly significant in chicken myoblasts.

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