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Updated: Jul 11, 2026

A Suppressor Screen for the Characterization of Genetic Links Regulating Chronological Lifespan in Saccharomyces cerevisiae
Published on: September 17, 2020
Integration of a pAL2-1 homologous mitochondrial plasmid associated with life span extension in Podospora anserina
M F P M Maas1, C H Sellem, R F Hoekstra
1CNRS, Centre de Génétique Moléculaire, 1 Avenue de la terrasse, 91198 Gif-sur-Yvette cedex, France. Maas@cgm.cnrs-gif.fr
Abstract:
We isolated and characterized a novel spontaneous longevity mutant of Podospora anserina strain Wa32 carrying one of the pAL2-1 homologous mitochondrial plasmids. This mutant is at least ten fold longer-lived than the wild type, and is hence a formal suppressor of both the regular and the 'plasmid-based' senescence process. We show that the longevity trait is maternally inherited and coincides with the presence of a copy of the plasmid integrated in the 5' UTR of the mitochondrial Complex I genes nd2 and nd3. This mutation is associated with complex alterations in the respiratory chain, including a dispensable induction of the alternative oxidase. It is also associated with a stabilization of the mitochondrial chromosome and a reduction of the overall cellular level of reactive oxygen species.
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