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Published on: December 10, 2007
Oligoclonal expansion and CD1 recognition by human intestinal intraepithelial lymphocytes
S P Balk1, E C Ebert, R L Blumenthal
1Hematology-Oncology Division, Beth Israel Hospital, Harvard Medical School, Boston, MA 02215.
Abstract:
A human intestinal intraepithelial lymphocyte (IEL) T cell line was established from jejunum to characterize the structure and function of the alpha beta T cell antigen receptors (TCRs) expressed by this population. Single-sided polymerase chain reaction (PCR) amplification cloning and quantitative PCR amplification of the TCR chains from the cell line and from fresh IELs demonstrated that IELs were oligoclonal. The IEL T cell line exhibited CD1-specific cytotoxicity and a dominant IEL T cell clone was CD1c-specific. Thus, human jejunal intraepithelial lymphocytes are oligoclonal and recognize members of the CD1 gene family.
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