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Related Experiment Video

Updated: Jul 18, 2026

Bilayer Microfluidic Device for Combinatorial Plug Production
07:03

Bilayer Microfluidic Device for Combinatorial Plug Production

Published on: December 1, 2023

Combinatorial dispensing as a fast and efficient means to create complex screens.

Bart Hazes1

  • 1Department of Medical Microbiology and Immunology, 1-15 Medical Sciences Building, University of Alberta, Edmonton, Alberta, Canada. Bart.Hazes@Ualberta.ca

Combinatorial Chemistry & High Throughput Screening
|December 16, 2006
PubMed
Summary

This study introduces a novel non-contact liquid handling system for creating complex reagent cocktails. This technology enables low-volume, high-density screening, enhancing efficiency in applications like protein crystallization.

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Area of Science:

  • Biotechnology
  • Automation
  • Chemical Engineering

Background:

  • Liquid handling robots are crucial for tasks ranging from simple plate filling to complex reagent cocktail creation.
  • General-purpose liquid handlers have limitations in exploiting the combinatorial nature of cocktail synthesis.
  • Current methods often require extensive tip washing or disposable tips, increasing costs and waste.

Purpose of the Study:

  • To present a novel approach using non-contact dispensing technologies for complex, low-volume, high-density screening.
  • To demonstrate the adaptability of this technology for diverse applications, including protein crystallization.
  • To address the limitations of conventional liquid handling systems in combinatorial processes.

Main Methods:

  • Utilizing an "inkjet printer principle" with a block of dispensers (print head) traveling over a multi-well plate.

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Achieving Efficient Fragment Screening at XChem Facility at Diamond Light Source
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Last Updated: Jul 18, 2026

Bilayer Microfluidic Device for Combinatorial Plug Production
07:03

Bilayer Microfluidic Device for Combinatorial Plug Production

Published on: December 1, 2023

Achieving Efficient Fragment Screening at XChem Facility at Diamond Light Source
08:35

Achieving Efficient Fragment Screening at XChem Facility at Diamond Light Source

Published on: May 29, 2021

  • Employing non-contact dispensing to deliver reagents in user-defined patterns.
  • Leveraging impact-induced mixing and eliminating tip contamination for reagent delivery.

    • Main Results:

    • Successful creation of complex screens at low volumes (50 nL to 50 µL) and high densities.
    • Demonstrated utility in protein crystallization screening, handling diverse reagent viscosities and surface tensions.
    • Elimination of the need for extensive tip washing or large numbers of disposable tips.

    Conclusions:

    • Non-contact dispensing technology offers an efficient and versatile solution for complex liquid handling tasks.
    • This approach significantly improves throughput and reduces waste in high-density screening applications.
    • The technology is well-suited for demanding applications like protein crystallization, accommodating a wide range of reagent properties and volumes.